Product Name	Histone H1.X
Chinese Name	组蛋白H1家族X抗体
Alias	H1 histone family member X; Histone H1fx; H1FX; H1X_HUMAN； H1X; Histone H1x; MGC15959; MGC8350.
Research Area	Cell biology  Epigenetics  Ubiquitin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	22kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Histone H1.X: 21-120/213
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008] Function: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. Subcellular Location: NuclearC. hromosome. Tissue Specificity: Expressed ubiquitously. Post-translational modifications: Citrullination at Arg-62 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance (By similarity). Similarity: Belongs to the histone H1/H5 family. Contains 1 H15 (linker histone H1/H5 globular) domain. SWISS: Q92522 Gene ID: 8971 Database links: Entrez Gene: 8971 Human Entrez Gene: 243529 Mouse Omim: 602785 Human SwissProt: Q92522 Human SwissProt: Q80ZM5 Mouse Unigene: 33796 Mouse
Product Picture	Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Histone H1.X (SL17424R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H1.X) Polyclonal Antibody, Unconjugated (SL17424R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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