TEL: +86 571 56623320 EMAIL: [email protected]
Product Name phospho-IRE1 (Ser724) Chinese Name 磷酸化IRE1蛋白抗体 Alias IRE1 (phospho S724); p-IRE1 (phospho S724); Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ER to nucleus signalling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring 1, S. cerevisiae, homolog of; Inositol requiring enzyme 1, S. cerevisiae, homolog of; Inositol requiring protein 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; MGC163279; Protein kinase/endoribonuclease; RGD1559716; Serine/threonine protein kinase/endoribonuclease IRE1. literatures Specific References (12) | SL16698R has been referenced in 12 publications.Research Area Cell biology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 107kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human IRE1 around the phosphorylation site of Ser724: RH(p-S)FS Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1α and IRE1β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes. In response to stress, sensors for the ER mammalian cells activate IRE1α and IRE1β, which then results in the phosphorylation of JNK (Jun N-Terminal Kinase) and the activation of the cellular MAP kinase pathway.
Function:
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Subcellular Location:
Endoplasmic reticulum membrane.
Tissue Specificity:
Ubiquitously expressed. High levels observed in pancreatic tissue.
Post-translational modifications:
Autophosphorylated.
Similarity:
Belongs to the protein kinase superfamily.
Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.
SWISS:
O75460
Gene ID:
2081
Database links:Entrez Gene: 10595 Human
Entrez Gene: 2081 Human
Entrez Gene: 78943 Mouse
Omim: 604033 Human
SwissProt: O75460 Human
SwissProt: Q76MJ5 Human
SwissProt: Q9EQY0 Mouse
SwissProt: Q9Z2E3 Mouse
Unigene: 133982 Human
Unigene: 592041 Human
Unigene: 700027 Human
Unigene: 20452 Mouse
Unigene: 340943 Mouse
Unigene: 226435 Rat
Product Picture Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-IRE1 (Ser724) (SL16698R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 112 kD
Sample:
Panc-1(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-IRE1 (Ser724) (SL16698R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kD
Observed band size: 107 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (SL16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (SL16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (SL16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Scan Wechat Qrcode