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Product Name phospho-PAK2 (Ser141) Chinese Name 磷酸化p21激活激酶2抗体 Alias PAK2 (phospho S141); p-PAK2 (phospho S141); C-t-PAK2; CB422; EC 2.7.11.1; Gamma PAK; Gamma-PAK; hPAK65; Kinase; p21 (CDKN1A) activated kinase 2; p21 (CDKN1A)-activated kinase 2a; p21 activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein Cdc42 Rac activated kinase 2; p21-activated kinase 2; p21-activated kinase, 65-KD; p21-activated protein kinase I; p21CDKN1A activated kinase 2; p27; p34; p58; p65PAK; PAK 2; PAK-2; PAK-2p34; PAK2_HUMAN; PAK65; PAKgamma; S6 H4 kinase; S6/H4 kinase; Serine threonine protein kinase PAK 2; Serine/threonine protein kinase PAK 2. literatures Specific References (1) | SL1654R has been referenced in 1 publications.[IF=5.07] Luo, Rui, et al. "Label-free quantitative phosphoproteomic analysis reveals differentially regulated proteins and pathway in PRRSV infected pulmonary alveolar macrophages." Journal of Proteome Research (2014). WB ; Pig.Product Type Phosphorylated anti Research Area Tumour Cell biology Microbiology Signal transduction Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 23/35/58kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human PAK2 around the phosphorylation site of Ser141: YM(p-S)FT Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008]
Function:
The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
Subcellular Location:
Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
Tissue Specificity:
Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
Post-translational modifications:
Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
Ubiquitinated, leading to its proteasomal degradation.
PAK-2p34 is myristoylated.
Similarity:
Belongs to the protein kinase superfamily.
STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
SWISS:
Q13177
Gene ID:
5062
Database links:Entrez Gene: 5062 Human
Entrez Gene: 224105 Mouse
GenBank: NP_002568.2 Human
Omim: 605022 Human
SwissProt: Q13177 Human
SwissProt: Q8CIN4 Mouse
Unigene: 518530 Human
Unigene: 234204 Mouse
PAK1-3蛋白具有广泛的生物学功能,也是一个保守的丝氨酸/苏氨酸蛋白激酶,参与许多重要的细胞活动。包括Cytoskeleton的动力学调节,细胞移动,生存和凋亡,细胞周期,基因转录调节,细胞生长Signal transduction和转化等。Product Picture Tissue/cell: Mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-p-PAK1 (Ser141) Polyclonal Antibody, Unconjugated(SL1654R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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