TEL: +86 571 56623320 EMAIL: [email protected]
Product Name phospho-HGS (Tyr334) Chinese Name 磷酸化肝细胞生长调节因子酪氨酸激酶底物抗体 Alias HGS (phospho Y334); p-HGS (phospho Y334); Hepatocyte growth factor regulated tyrosine kinase substrate; Hepatocyte growth factor-regulated tyrosine kinase substrate; HGF-regulated tyrosine kinase substrate; HGNC:4897; HGS; HGS_HUMAN; HRS; Human growth factor regulated tyrosine kinase substrate; Protein pp110; ZFYVE8. Product Type Phosphorylated anti Research Area Cell biology immunology Signal transduction Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 86kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human HGS around the phosphorylation site of Tyr334: RN(p-Y)WE Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail HGS is a regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010].
Function:
Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.
Subcellular Location:
Cytoplasm. Early endosome membrane. Endosome,multivesicular body membrane.
Tissue Specificity:
Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes.
Post-translational modifications:
Phosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected. Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF. Ubiquitinated by ITCH.
Similarity:
Contains 1 FYVE-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain.
SWISS:
O14964
Gene ID:
9146
Database links:Entrez Gene: 9146 Human
Entrez Gene: 15239 Mouse
Omim: 604375 Human
SwissProt: O14964 Human
SwissProt: Q99LI8 Mouse
Unigene: 514590 Human
Unigene: 7919 Mouse
Scan Wechat Qrcode