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Product Name ZRANB3 Chinese Name ZRANB3蛋白抗体 Alias 4933425L19Rik; MGC105033; MGC75012; OTTHUMP00000197449; Zinc finger Ran-binding domain-containing protein 3; Zinc finger, RAN- binding domain containing 3; ZRAB3_HUMAN; Zranb3. Research Area transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 123kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ZRANB3: 981-1079/1079 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Zinc finger Ran-binding domain-containing protein 3 (ZRANB3) is a 1079 amino acid gene product that belongs to the SNF2/RAD54 helicase family. Other family members are known to be involved in DNA repair and mitotic recombination. This family of proteins often plays an active role in recombination processes in cooperation with other members of the RAD52 epistasis group. The ZRANB3 product contains one helicase ATP-binding domain, one helicase C-terminal domain and one RanBP2-type zinc finger. RanBP2-type zinc fingers mediate the helicase binding to RNA. Similar to other Ran-binding zinc finger proteins, ZRANB3 likely acts as a splice factor for alternative splicing events of pre-mRNA transcripts. ZRANB proteins may also interfere with constitutive 5'-splice site selection.
Function:
DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks.
Subunit:
Interacts (via PIP-box and RanBP2-type zinc finger) with PCNA (when PCNA is polyubiquitinated via 'Lys-63'-linked polyubiquitin).
Subcellular Location:
Nucleus. Chromosome. Note=Following DNA damage, recruited to sites of DNA damage and stalled replication forks by polyubiquitinated PCNA.
Similarity:
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 RanBP2-type zinc finger.
SWISS:
Q5FWF4
Gene ID:
84083
Database links:Entrez Gene: 84083 Human
SwissProt: Q5FWF4 Human
Unigene: 658422 Human
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