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Product Name phospho-GRLF1 (Tyr1087) Chinese Name 磷酸化糖皮质激素受体DNA结合因子1抗体 Alias GRLF1 (phospho Y1087); p-GRLF1 (phospho Tyr1087); ARHGAP35; GAP associated protein p190; Glucocorticoid receptor DNA binding factor 1; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; Glucocorticoid receptor repression factor 1; GRF 1; GRF-1; GRF1; GRLF 1; GRLF1; GRLF1_HUMAN; KIAA1722; MGC10745; p190 A; p190-A; P190A; P190A, rat, homolog of; p190ARhoGAP; p190RhoGAP; Rho GAP p190A; Rho GAP p190A; Rho GTPase-activating protein 35. Product Type Phosphorylated anti Research Area Cell biology transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 171kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human GRLF1 around the phosphorylation site of Tyr1087: SD(p-Y)AE Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]
Function:
Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
Subcellular Location:
Cytoplasm. Nucleus.
Post-translational modifications:
Tyrosine phosphorylated.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Contains 4 FF domains.
Contains 1 Rho-GAP domain.
SWISS:
Q9NRY4
Gene ID:
2909
Database links:Entrez Gene: 2909 Human
Entrez Gene: 232906 Mouse
Omim: 605277 Human
SwissProt: Q9NRY4 Human
SwissProt: Q91YM2 Mouse
Unigene: 509447 Human
Unigene: 28646 Mouse
Unigene: 400358 Mouse
Unigene: 16508 Rat
Unigene: 222475 Rat
Product Picture Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-GRLF1 (Tyr1087) Polyclonal Antibody, Unconjugated(SL16322R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-GRLF1 (Tyr1087) Polyclonal Antibody, Unconjugated(SL16322R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-phospho-GRLF1(Tyr1087) antibody (SL16322R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed
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