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Product Name FUCA2 Chinese Name α-L岩藻糖苷酶2抗体 Alias Alpha L fucosidase 2; Alpha L fucosidase fucohydrolase 2; Alpha-L-fucosidase 2; Alpha-L-fucoside fucohydrolase 2; dJ20N2.5; FUCA 2; FUCA2; FUCO2; FUCO2_HUMAN; Fucosidase alpha L 2 plasma; MGC1314; Plasma alpha L fucosidase; Plasma alpha-L-fucosidase; RP1 20N2.5. literatures Specific References (1) | SL16192R has been referenced in 1 publications.[IF=5.432] Dongming Liu. et al. Diagnostic value of 5 serum biomarkers for hepatocellular carcinoma with different epidemiological backgrounds: A large-scale, retrospective study. Cancer Biol Med. 2021 Feb 15; 18(1): 256–270 IHC ; Human.Research Area Cell biology Signal transduction The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Chicken, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 51kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FUCA2: 331-430/467 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. The protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. This enzyme is essential for Helicobacter pylori adhesion to human gastric cancer cells. [provided by RefSeq, Aug 2010]
Function:
Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.
Subcellular Location:
Secreted.
Similarity:
Belongs to the glycosyl hydrolase 29 family.
SWISS:
Q9BTY2
Gene ID:
2519
Database links:Entrez Gene: 2519 Human
Entrez Gene: 100174203 Orangutan
Omim: 136820 Human
SwissProt: Q9BTY2 Human
SwissProt: Q5RFI5 Orangutan
Unigene: 17680 Human
Product Picture Protein: heart(mouse) lysate at 40ug;
Primary: rabbit Anti-FUCA2(SL16192R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000;
Predicted band size: 51 kD
Observed band size: 51 kDTissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-FUCA2 Polyclonal Antibody, Unconjugated(SL16192R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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