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Rabbit Anti-FUCA2 antibody
Rabbit Anti-FUCA2 antibody
Alpha L fucosidase 2; Alpha L fucosidase fucohydrolase 2; Alpha-L-fucosidase 2; Alpha-L-fucoside fucohydrolase 2; dJ20N2.5; FUCA 2; FUCA2; FUCO2; FUCO2_HUMAN; Fucosidase alpha L 2 plasma; MGC1314; Plasma alpha L fucosidase; Plasma alpha-L-fucosidase; RP1
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  • NO.:SL16192R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,Mouse,(predicted: Rat,Chicken,Cow,Horse,Sheep,)
    Applications:WB ELISA IHC-P IHC-F ICC IF
    concentration:1mg/ml
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Details

Product Name FUCA2
Chinese Name α-L岩藻糖苷酶2抗体
Alias Alpha L fucosidase 2; Alpha L fucosidase fucohydrolase 2; Alpha-L-fucosidase 2; Alpha-L-fucoside fucohydrolase 2; dJ20N2.5; FUCA 2; FUCA2; FUCO2; FUCO2_HUMAN; Fucosidase alpha L 2 plasma; MGC1314; Plasma alpha L fucosidase; Plasma alpha-L-fucosidase; RP1 20N2.5.  
literatures
Specific References  (1)     |     SL16192R has been referenced in 1 publications.
[IF=5.432] Dongming Liu. et al. Diagnostic value of 5 serum biomarkers for hepatocellular carcinoma with different epidemiological backgrounds: A large-scale, retrospective study. Cancer Biol Med. 2021 Feb 15; 18(1): 256–270  IHC ;  Human.  
Research Area Cell biology  Signal transduction  The new supersedes the old  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human, Mouse,  (predicted: Rat, Chicken, Cow, Horse, Sheep, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 51kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human FUCA2: 331-430/467 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail This gene encodes a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. The protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. This enzyme is essential for Helicobacter pylori adhesion to human gastric cancer cells. [provided by RefSeq, Aug 2010]

Function:
Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.

Subcellular Location:
Secreted.

Similarity:
Belongs to the glycosyl hydrolase 29 family.

SWISS:
Q9BTY2

Gene ID:
2519

Database links:

Entrez Gene: 2519 Human

Entrez Gene: 100174203 Orangutan

Omim: 136820 Human

SwissProt: Q9BTY2 Human

SwissProt: Q5RFI5 Orangutan

Unigene: 17680 Human



Product Picture
Protein: heart(mouse) lysate at 40ug;
Primary: rabbit Anti-FUCA2(SL16192R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000;
Predicted band size: 51 kD
Observed band size: 51 kD
Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-FUCA2 Polyclonal Antibody, Unconjugated(SL16192R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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