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Product Name CDH17/LI Cadherin Chinese Name 肝肠钙粘连蛋白抗体 Alias CAD17_HUMAN; Cadherin-17; CDH17; Intestinal peptide-associated transporter HPT-1; Liver-intestine cadherin (LI-cadherin); HPT1; CDH16; HPT-1; Research Area immunology Cytoskeleton Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 90kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human LI-cadherin: 101-200/832 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]
Function:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein (Potential).
Tissue Specificity:
Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin.
Similarity:
Contains 7 cadherin domains.
SWISS:
Q12864
Gene ID:
1015
Database links:Entrez Gene: 1015 Human
Omim: 603017 Human
SwissProt: Q12864 Human
Unigene: 591853 Human
肝肠钙粘连蛋白是钙粘连蛋白超家族中的特殊一员,在结构上与经典Ⅰ型钙粘连蛋白有所不同而被划分为经典钙粘连蛋白的变异体,主要用于消化道Tumour方面的研究。Product Picture Sample:
SW480(Human) Cell Lysate at 30 ug
Primary: Anti-LI Cadherin (SL1617R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 130 kD
Sample:
Lane 1: Human FHC cell Lysates
Lane 2: Human LOVO cell Lysates
Lane 3: Human SW480 cell Lysates
Primary: Anti-LI Cadherin(SL1617R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90kDa
Observed band size: 130kDa
Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LI Cadherin) Polyclonal Antibody, Unconjugated (SL1617R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LI Cadherin) Polyclonal Antibody, Unconjugated (SL1617R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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