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Rabbit Anti-FOXB2 antibody
Rabbit Anti-FOXB2 antibody
bA159H20.4; Forkhead box B2; Forkhead box protein B2; FOXB 2; Foxb2; FOXB2_HUMAN.
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  • NO.:SL16161R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,Mouse,(predicted: Rat,Cow,Rabbit,)
    Applications:WB IHC-P IHC-F ICC IF
    concentration:1mg/ml
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Product Name FOXB2
Chinese Name 叉头蛋白B2抗体
Alias bA159H20.4; Forkhead box B2; Forkhead box protein B2; FOXB 2; Foxb2; FOXB2_HUMAN.  
Research Area Cell biology  Signal transduction  transcriptional regulatory factor  Epigenetics  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human, Mouse,  (predicted: Rat, Cow, Rabbit, )
Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 45kDa
Cellular localization The nucleus 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human FOXB2: 351-432/432 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The Forkhead-box (FOX) genes comprise a superfamily of at least 43 members that encode proteins which are involved in transcriptional regulation and may be associated with the pathogenesis of various cancers. FOXB1 (forkhead box B1), also known as FKH5 or HFKH-5, and FOXB2 (forkhead box B2) are members of the FOX family and each contain one forkhead DNA-binding domain. Both FOXB1 and FOXB2 localize to the nucleus where they are thought to function as transcription factors that can bind to DNA via their forkhead domains. In mice, defects in the gene encoding FOXB1 are associated with retarded development of the central nervous system (CNS), suggesting that FOXB1 may play a role in CNS organization and function.

Function:
Transcription factor.

Subcellular Location:
Nucleus.

Similarity:
Contains 1 fork-head DNA-binding domain.

SWISS:
Q5VYV0

Gene ID:
442425

Database links:

Entrez Gene: 442425 Human

Entrez Gene: 14240 Mouse

Entrez Gene: 691398 Rat

SwissProt: Q5VYV0 Human

SwissProt: Q64733 Mouse

Unigene: 553843 Human

Unigene: 7971 Mouse



Product Picture
Sample:
HepG2 Cell (Human) Lysate at 40 ug
Hcclm3 Cell (Human) Lysate at 40 ug
Primary: Anti-FOXB2 (SL16161R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse embryo); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FOXB2) Polyclonal Antibody, Unconjugated (SL16161R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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