Product Name	FITM1
Chinese Name	FITM1蛋白抗体
Alias	Fat inducing protein 1; Fat inducing transcript 1; Fat storage inducing transmembrane protein 1; Fat storage-inducing transmembrane protein 1; Fat-inducing protein 1; FIT1; FITM 1; Fitm1; FITM1_HUMAN; MGC46490.
Research Area	Cell biology  immunology  Signal transduction  Cell type markers
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	32kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FITM1: 1-100/292
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	FIT1 belongs to an evolutionarily conserved family of proteins involved in fat storage (Kadereit et al., 2008 [PubMed 18160536]).[supplied by OMIM, May 2008] Function: Plays an important role in lipid droplet accumulation. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Primarily expressed in heart and skeletal muscle. Similarity: Belongs to the FIT family. SWISS: A5D6W6 Gene ID: 161247 Database links: Entrez Gene: 161247 Human Entrez Gene: 68680 Mouse Omim: 612028 Human SwissProt: A7YWN2 Cow SwissProt: A5D6W6 Human SwissProt: Q91V79 Mouse SwissProt: B2MVP8 Pig Unigene: 128060 Human Unigene: 36758 Mouse
Product Picture	Protein: 293T(human) lysate at 40ug; Primary: rabbit Anti-FITM1 (SL16090R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size: 32 kD Observed band size: 28 kD Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (SL16090R) at 1:400 overnight at 4∑C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (FITM1) Polyclonal Antibody, Unconjugated (SL16090R) at 1:400 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Fat inducing transcript; FITM1) Polyclonal Antibody, Unconjugated (SL16090R) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.

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