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Product Name FBXO10 Chinese Name FBXO10蛋白抗体 Alias F box protein 10; F-box only protein 10; FBX10; FBX10_HUMAN; Fbxo10; hCG_2011110; PRMT11. Research Area Cell biology Signal transduction Epigenetics Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 105kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FBXO10: 21-120/956 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Members of the F-box protein family, such as FBXO10, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]
Similarity:
Contains 1 F-box domain.
Contains 17 PbH1 repeats.
SWISS:
Q9UK96
Gene ID:
26267
Database links:Entrez Gene: 26267 Human
Entrez Gene: 269529 Mouse
Omim: 609092 Human
SwissProt: Q9UK96 Human
SwissProt: Q7TQF2 Mouse
Unigene: 709527 Human
Unigene: 389552 Mouse
Unigene: 223157 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FBXO10) Polyclonal Antibody, Unconjugated (SL16045R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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