Product Name	Bcl-2 alpha
Chinese Name	Bcl2 alpha蛋白抗体
Alias	Apoptosis regulator Bcl2; B cell CLL; B cell lymphoma 2; B cell lymphoma protein 2 alpha isoform; B cell lymphoma protein 2; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; bcl2a; Leukemia /lymphoma, B-cell, 2; Oncogene B cell leukemia 2; Oncogene B-cell leukemia 2.
literatures	Specific References  (1)     |     SL15533R has been referenced in 1 publications. [IF=3.063] Mengmeng Yu. et al. Taurine attenuates gossypol-induced apoptosis of C2C12 mouse myoblasts via the GPR87-AMPK/AKT signaling. Amino Acids. 2020 Sep;52(9):1285-1298  WB ;  Mouse.   PubMed:32918616
Research Area	Tumour  Cell biology  immunology  Signal transduction  Apoptosis  The new supersedes the old  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	26kDa
Cellular localization	The nucleus cytoplasmic Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Bcl-2 alpha: 51-150/239
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]. SWISS: P49950 Gene ID: 596 Database links: Entrez Gene: 596 Human Omim: 151430 Human SwissProt: P10415 Human Unigene: 150749 Human GenBank: ABX60202
Product Picture	Sample: Lane 1: Recombinant human Bcl-2 protein, C-His Primary: Anti- Bcl-2 alpha (SL15533R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Bcl-2 Polyclonal Antibody, Unconjugated(SL15533R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control:HL-60. Primary Antibody (green line): Rabbit Anti-Bcl-2 alpha antibody (SL15533R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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