Product Name	GPR89A
Chinese Name	G protein-coupled receptorGPR89A蛋白抗体
Alias	CGI-13; Golgi pH regulator A; Golgi pH regulator B; GPHR; GPHRA; GPHRB; GPR89; GPR89A; GPR89B; HSPC201; Protein GPR89A; Protein GPR89B; Putative MAPK-activating protein PM01; Putative NF-kappa-B-activating protein 90; SH120; UNQ192/PRO218; GPHRA_HUMAN.
Research Area	Cell biology  immunology  Signal transduction  G protein-coupled receptor  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	53kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GPR89A: 221-320/445
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GPR89A is a nearly identical copy of the GPR89B gene (MIM612806). Function: Voltage dependent anion channel required for acidification and functions of the Golgi apparatus that may function in counter-ion conductance. Subunit: Homotrimer. Subcellular Location: Golgi apparatus membrane; Multi-pass membrane protein. Tissue Specificity: Ubiquitous. Similarity: Belongs to the Golgi pH regulator (TC 1.A.38) family. SWISS: B7ZAQ6 Gene ID: 653519 Database links: Entrez Gene: 653519 Human Entrez Gene: 67549 Mouse Entrez Gene: 362003 Rat Omim: 612821 Human SwissProt: B7ZAQ6 Human SwissProt: Q8BS95 Mouse
Product Picture	Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Rat Cerebrum tissue lysates Primary: Anti-GPR89A (SL15391R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kDa Observed band size: 50 kDa Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR89A) Polyclonal Antibody, Unconjugated (SL15391R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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