Product Name	COX4I1
Chinese Name	细胞色素c氧化酶IV亚型1抗体
Alias	COX41_HUMAN; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1 (COX IV-1); COX-IV; COX IV; COX 4; COX 4I1;
literatures	Specific References  (6)     |     SL1533R has been referenced in 6 publications. [IF=6.551] Wei J et al. Endosulfan induces cardiotoxicity through apoptosis via unbalance of pro-survival and mitochondrial-mediated apoptotic pathways. Sci Total Environ . 2020 Jul 20;727:138790.  WB ;  human.   PubMed:32344260 [IF=6.025] Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078  WB ;  Mouse.   PubMed:35489469 [IF=4.848] Delong Wang. et al. Baoyuan Jiedu Decoction Alleviates Cancer-Induced Myotube Atrophy by Regulating Mitochondrial Dynamics Through p38 MAPK/PGC-1α Signaling Pathway. Front Oncol. 2020; 10: 523577  WB ;  Mouse.   PubMed:33102208 [IF=4.451] Jie Gao. et al. Ferulic acid targets ACSL1 to ameliorate lipid metabolic disorders in db/db mice. J Funct Foods. 2022 Apr;91:105009  WB ;  Human.   PubMed:10.1016/j.jff.2022.105009 [IF=3.266] Zhang H et al. Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death. Am J Transl Res. 2019 Jul 15;11(7):4568-4583.  WB ;  Rat&Human.   PubMed:31396361 [IF=2.238] Qiao Z et al. Casticin inhibits esophageal cancer cell proliferation and promotes apoptosis by regulating mitochondrial apoptotic and JNK signaling pathways.(2018)Naunyn Schmiedebergs Arch Pharmacol.Nov 17  WB ;  Human.   PubMed:30448926
Research Area	Cell biology  immunology  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Cellular localization	cytoplasmic The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human COX4I1: 101-169/169
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008] Function: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Subcellular Location: Mitochondrion inner membrane. Tissue Specificity: Ubiquitous. Similarity: Belongs to the cytochrome c oxidase IV family. SWISS: P13073 Gene ID: 1327 Database links: Entrez Gene: 1327 Human Entrez Gene: 12857 Mouse Entrez Gene: 29445 Rat SwissProt: P13073 Human SwissProt: P19783 Mouse SwissProt: P10888 Rat 细胞色素c氧化酶4亚型1抗体；（细胞色素c氧化酶IV亚型1）是一种存在Mitochondrion上的大型Transmembrane protein复合物。由于细胞色素氧化酶是呼吸电子传递链的第四个中心酶复合物，因此又被称为复合物IV。
Product Picture	Sample: Lane 1: Human Raji cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human MCF-7 cell lysates Lane 4: Human A431 cell lysates Primary: Anti-COX4I1 (SL1533R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 15 kDa Sample: kidney (Mouse) Lysate at 40 ug Primary: Anti-COX4 (Bs-1533R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kD Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-COX4 (Bs-1533R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kD Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-COX4/COX IV-1 Polyclonal Antibody, Unconjugated(SL1533R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Positive control: Hela cells Concebtration: 5μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice.

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