Product Name	CD63
Chinese Name	黑色素瘤相关抗原抗体
Alias	Lysosomal associated membrane protein 3; CD63 antigen; CD63 antigen melanoma 1 antigen; CD63 molecule; granulophysin; LAMP 3; LAMP3; lysosome associated membrane glycoprotein 3; CD 63; CD63 antigen (melanoma 1 antigen); CD63_HUMAN; gp55; granulophysin; LAMP-3; LIMP; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; NGA; Ocular melanoma-associated antigen; PTLGP40; Tetraspanin-30; Tspan-30; Mast cell antigen AD1; ME491; melanoma 1 antigen; Melanoma associated antigen ME491; Melanoma associated antigen MLA1; MGC72893; MLA 1; MLA1; ocular melanoma associated antigen; OMA81H; Tetraspanin 30; Tspan 30; TSPAN30; Tumor biomarkers; Platelets markers.
literatures	Specific References  (15)     |     SL1523R has been referenced in 15 publications. [IF=10.435] Li, Kanglu. et al. Anti-inflammatory and immunomodulatory effects of the extracellular vesicles derived from human umbilical cord mesenchymal stem cells on osteoarthritis via M2 macrophages. J Nanobiotechnol. 2022 Dec;20(1):1-20  WB ;  Human.   PubMed:35057811 [IF=9.598] Sung MS et al. Single-Molecule Co-Immunoprecipitation Reveals Functional Inheritance of EGFRs in Extracellular Vesicles. (2018) Small,14(42):e1802358.  FCM WB ;   PubMed:30239124 [IF=7.711] Xiaoyi Gao. et al. Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins. Acs Sensors. 2021;6(10):3611–3620  FC ;  Human.   PubMed:34632781 [IF=7.31] Jingsong Chen. et al. Exploring the Temporal Correlation of Sarcopenia with Bone Mineral Density and the Effects of Osteoblast-Derived Exosomes on Myoblasts through an Oxidative Stress-Related Gene.. OXID MED CELL LONGEV. 2022 Sep;2022:9774570-9774570  WB ;  Mouse.   PubMed:36160702 [IF=5.58] Marzesco, Anne-Marie, et al. "Highly potent intracellular membrane-associated Aβ seeds." Scientific Reports 6 (2016): 28125.  WB, other ;  Mouse.   PubMed:27311744 [IF=4.757] Yessenia L. Molina. et al. Rottlerin Stimulates Exosome/Microvesicle Release Via the Increase of Ceramide Levels Mediated by Ampk in an In Vitro Model of Intracellular Lipid Accumulation. BIOMEDICINES. 2022 Jun;10(6):1316  WB ;  Rat.   PubMed:35740338 [IF=4.556] Lorena del Pozo-Acebo. et al. Bovine Milk-Derived Exosomes as a Drug Delivery Vehicle for miRNA-Based Therapy. Int J Mol Sci. 2021 Jan;22(3):1105  WB ;  Bovine.   PubMed:33499350 [IF=4.402] García-Seisdedos D et al. Curcumin stimulates exosome/microvesicle release in an in vitro model of intracellular lipid accumulation by increasing ceramide synthesis. Biochim Biophys Acta Mol Cell Biol Lipids. 2020 May;1865(5):158638.  WB&FCM ;  Rat.   PubMed:31988047 [IF=3.998] Jik-Han Jung. et al. Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma. Sci Rep-Uk. 2021; 11: 217  WB ;  Human.   PubMed:33436891 [IF=3.829] Wang Ruibiao. et al. Effects of exosomes derived from Trichinella spiralis infective larvae on intestinal epithelial barrier function. VET RES. 2022 Dec;53(1):1-11  WB ;  Pig.   PubMed:36273217 [IF=3.811] Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18.  WB ;  Human&Mouse.   PubMed:31853695 [IF=3.471] Höglund Nina. et al. Isolation of Extracellular Vesicles From the Bronchoalveolar Lavage Fluid of Healthy and Asthmatic Horses. FRONT VET SCI. 2022 Jun;0:853  WB ;  Horse.   PubMed:35799843 [IF=2.389] Huang Weizhen. et al. LncRNA SNHG11 enhances bevacizumab resistance in colorectal cancer by mediating miR-1207-5p/ABCC1 axis. ANTI-CANCER DRUG. 2022 Jul;33(6):575-586  WB ;  Human.   PubMed:35324517 [IF=0] Wang CJ et al. Exosome‑delivered TRPP2 siRNA inhibits the epithelial‑mesenchymal transition of FaDu cells. Oncol Lett. 2019 Feb;17(2):1953-1961.  WB ;  Human.   PubMed:30675260 [IF=0] Sun, Weijia, et al. "Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity." Cell Discovery 2 (2016).  WB ;  Mouse.   PubMed:27462462
Research Area	Tumour  Cardiovascular  immunology  Cell type markers
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	26kDa
Detection molecular weight	30-50 kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD63: 101-200/238 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012] Function: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. Subcellular Location: Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. Tissue Specificity: Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. Similarity: Belongs to the tetraspanin (TM4SF) family. SWISS: P08962 Gene ID: 967 Database links: Entrez Gene: 967 Human Omim: 155740 Human SwissProt: P08962 Human Unigene: 445570 Human CD63为溶酶体膜glycoprotein也是一种细胞表面glycoprotein，可表达在多种细胞的表面和胞浆内，血小板活化后即转移至膜表面。 CD63—又称黑色素瘤相关抗原MLA1和整合素形成复合物。是血液血小板活化的标志。与一些Tumour的进展也有关联。
Product Picture	Sample: A431 cell(human) Lysate at 40 ug HL-60 cell(human) Lysate at 40 ug MCF-7 cell(human) Lysate at 40 ug U87mg cell(human)Lysate at 40 ug Primary: Anti- CD63 (SL1523R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26kD Observed band size: 53 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD63 Polyclonal Antibody, Unconjugated(SL1523R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining cell: A549 cells Incubation: Avoid light, at 4°C for 60 minutes. Blank control A549 cells(red line),5X10^5/ml, at 4°C for 60 minutes. primary antibody: abbit Anti-CD63 antibody (SL1523R-PE,Blue line) ,1:50, at 4°C for 60 minutes. Blank control: Hep G2 cells(blue). Primary Antibody:Rabbit Anti- CD23 antibody(SL1523R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (SL1523R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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