Product Name	C3orf58
Chinese Name	3号染色体开放阅读框58抗体
Alias	Chromosome 3 open reading frame 58; DIA1; Golgi protein GoPro49; MGC33365; UPF0672 protein C3orf58; DIA1_HUMAN.
Research Area	Tumour  Cardiovascular  Cell biology  immunology  Developmental biology  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Cow, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	49kDa
Cellular localization	cytoplasmic Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human C3orf58: 351-430/430
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	C3orf58 (chromosome 3 open reading frame 58), also known as DIA1, is a 430 amino acid secreted protein that belongs to the UPF0672 family. C3orf58 is encoded by a gene that maps to human chromosome 3q24. Chromosome 3 is made up of approximately 214 million bases encoding over 1,100 genes. Notably, there is a chemokine receptor gene cluster and a variety of human cancer related loci on chromosome 3. Particular regions of the chromosome 3 short arm are deleted in many types of cancer cells. Key tumor suppressing genes on chromosome 3 encode apoptosis mediator RASSF1, cell migration regulator HYAL1 and angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth disease are a few of the numerous genetic diseases associated with chromosome 3. SWISS: Q8NDZ4 Gene ID: 205428 Database links: Entrez Gene: 205428 Human Omim: 612200 Human SwissProt: Q8NDZ4 Human
Product Picture	Sample: Lane 1: Mouse Colon tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Rat Colon tissue lysates Lane 4: Human 293T cell lysates Lane 5: Human U251 cell lysates Primary: Anti-C3orf58 (SL15179R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 45 kDa Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C3orf58) Polyclonal Antibody, Unconjugated (SL15179R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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