Product Name	EXDL1
Chinese Name	EXDL1蛋白抗体
Alias	exd1; EXD1_HUMAN; MGC33637; OTTHUMP00000160721.
Research Area	Cell biology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	58kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human EXDL1: 101-200/514
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	EXDL1 is a 514 amino acid protein that belongs to the EXD1 family and contains one 3'-5' exonuclease domain. Existing as two alternatively spliced isoforms, the gene encoding EXDL1 maps to human chromosome 15q15.1 and mouse chromosome 2 E5. Encoding more than 700 genes, chromosome 15 is made up of approximately 106 million base pairs and is about 3% of the human genome. Angelman and Prader-Willi syndromes are associated with loss of function or deletion of genes in the 15q11-q13 region. In the case of Angelman syndrome, this loss is due to inactivity of the maternal 15q11-q13 encoded UBE3A gene in the brain by either chromosomal deletion or mutation. In cases of Prader-Willi syndrome, there is a partial or complete deletion of this region from the paternal copy of chromosome 15. Similarity: Belongs to the EXD1 family. Contains 1 3'-5' exonuclease domain. SWISS: Q8NHP7 Gene ID: 161829 Database links: Entrez Gene: 161829 Human SwissProt: Q8NHP7 Human Unigene: 307999 Human
Product Picture	Sample: A549 (Human) Cell Lysate at 40 ug Primary: Anti-EXDL1(SL14656R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 52 kD Sample:HT29 (Human) Cell Lysate at 40 ug Primary: Anti-EXDL1(SL14656R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD Sample: Raji Cell (Human) Lysate at 40 ug Testis (Mouse) Lysate at 40 ug Primary: Anti-EXDL1 (SL14656R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD Sample: 293T (Human)Cell Lysate at 40 ug Primary: Anti-EXDL1(SL14656R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXDL1) Polyclonal Antibody, Unconjugated (SL14656R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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