TEL: +86 571 56623320 EMAIL: [email protected]
Product Name EXD2 Chinese Name EXD2蛋白抗体 Alias 4930539P14Rik; C14orf114; C85658; DKFZp781A0133; DKFZp781L15100; Exd2; EXD2_HUMAN; EXDL2; Exonuclease 3''-5'' domain-containing protein 2; Exonuclease 3''-5'' domain-like-containing protein 2; exonuclease 3'-5' domain-like 2; FLJ10738; RGD1311087. Research Area Cell biology Signal transduction Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 70kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human EXD2: 351-621/621 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture Sample: Lung(Mouse)Lysate at 40 ug
Primary: Anti-EXD2(SL14654R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 70kD
Observed band size: 56kD
Sample: HepG2 (Human)Cell Lysate at 40 ug
Primary: Anti-EXD2(SL14654R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 70kD
Observed band size: 56kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXD2) Polyclonal Antibody, Unconjugated (SL14654R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Scan Wechat Qrcode