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Product Name IL-12 Chinese Name 白介素12抗体 Alias Interleukin-12 subunit alpha; IL-12; CLMF p35; CLMF1; CTL maturation factor (TcMF); Cytotoxic lymphocyte maturation factor 1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL 12 subunit p35; IL12A; Interleukin 12 alpha chain; Interleukin 12 p35; Interleukin 12A; Natural killer cell stimulatory factor 1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1; IL-12A; IL12A_HUMAN; IL12A_RAT. Research Area Tumour Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 22kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from rat IL-12: 141-200/215 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail IL-12 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
Function:
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Subunit:
Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12.
Subcellular Location:
Secreted.
Similarity:
Belongs to the IL-6 superfamily.
SWISS:
P29459
Gene ID:
84405
Database links:Entrez Gene: 3592 Human
Entrez Gene: 16159 Mouse
Omim: 161560 Human
SwissProt: P29459 Human
SwissProt: P43431 Mouse
Unigene: 673 Human
Unigene: 103783 Mouse
Unigene: 207199 Rat
IL-12是新近发现的cell factor,具有多种生物学活性,尤其是在抗Tumour免疫和抗病毒免疫中有重要的作用。Product Picture Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti-IL-12 (SL14637R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 24 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-12) Polyclonal Antibody, Unconjugated (SL14637R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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