Product Name	VDAC (Mitochondrial Loading Control)
Chinese Name	等电压依赖性阴离子通道（内参）抗体
Alias	voltage-dependent anion channel; hVDAC1; MGC111064; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31 HL; Porin 31HL; Porin 31HM; VDAC 1; VDAC; VDAC1; Voltage dependent anion channel 1; Voltage dependent anion selective channel protein 1; VDAC1_HUMAN.
literatures	Specific References  (7)     |     SL1461R has been referenced in 7 publications. [IF=7.69] Manczak, Maria, and P. Hemachandra Reddy. "Abnormal interaction of VDAC1 with amyloid beta and phosphorylated tau causes mitochondrial dysfunction in Alzheimer's disease." Human molecular genetics 21.23 (2012): 5131-5146.  WB, IP ;  Human, Mouse.   PubMed:22926141 [IF=6.575] Qiyu Gan. et al. Prognostic Value and Immune Infiltration of HPV-Related Genes in the Immune Microenvironment of Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma. CANCERS. 2023 Jan;15(5):1419  IHC ;  Human.   PubMed:36900213 [IF=5.19] Peng Junjun. et al. Mitochondria-associated endoplasmic reticulum membranes participate mitochondrial dysfunction and endoplasmic reticulum stress caused by copper in duck kidney. ENVIRON SCI POLLUT R. 2023 May;:1-12  WB ;  Duck.   PubMed:37253910 [IF=2.73] Wang, Zhaoqi, et al. "Penehyclidine hydrochloride prevents anoxia/reoxygenation injury and induces H9c2 cardiomyocyte apoptosis via a mitochondrial pathway." European Journal of Pharmacology (2017).  WB ;  Rat.   PubMed:28089921 [IF=2.08] Xing, Wen Min, et al. "Proteomic identification of mitochondrial targets involved in andrographolide sodium bisulfite-induced nephrotoxicity in a rat model."Environmental Toxicology and Pharmacology (2015).  WB ;  Rat.   PubMed:26356389 [IF=1.35] Hui, Yan, et al. "Resveratrol improves mitochondrial function in the remnant kidney from 5/6 nephrectomized rats." Acta Histochemica (2017).  WB ;  Rat.   PubMed:28434671 [IF=1.26] Tan, Hongbao, Li Chen, and Jun Ma. "Penehyclidine hydrochloride post‑conditioning reduces ischemia/reperfusion‑induced cardiomyocyte apoptosis in rats." Experimental and Therapeutic Medicine.  WB ;  Rat.   PubMed:29104640
Product Type	Internal reference anti
Research Area	Cell biology  immunology  Apoptosis  Channel protein  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	32kDa
Cellular localization	cytoplasmic The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human VDAC: 85-190/283
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Voltage dependent anion selective channel protein 1 (VDAC/Porin) belongs to the eukaryotic mitochondrial porin family and forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel allows diffusion of small hydrophilic molecules; it adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation selective. VDAC/Porin expression is observed in the heart,liver and skeletal muscle. Function: Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (By similarity). Involved in HIV-1 budding. Subunit: Interacts with VPS4B. Interacts with CHMP1B. Interacts with CHMP2A; the interaction probably involves the open conformation of (polymerized) CHMP2A. May interact with CHMP3. Interacts with CHMP5; the interaction involves soluble CHMP5. Interacts with IST1. Subcellular Location: Cytoplasm. Endosome membrane; Peripheral membrane protein (Probable). Similarity: Belongs to the VTA1 family. SWISS: P21796 Gene ID: 7416 Database links: Entrez Gene: 416320 Chicken Entrez Gene: 282119 Cow Entrez Gene: 7416 Human Entrez Gene: 22333 Mouse Entrez Gene: 397010 Pig Entrez Gene: 83529 Rat Entrez Gene: 334582 Zebrafish Omim: 604492 Human 电压依赖性阴离子通道VDAC是存在于Mitochondrion外膜上的31kDa蛋白,能在膜上形成亲水性通道,调控阴离子、阳离子、ATP以及其他代谢物进出Mitochondrion,在调节细胞代谢、维持胞内钙稳态,调节Apoptosis和坏死等过程中发挥重要功能。
Product Picture	Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-VDAC (SL1461R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 25 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VDAC Polyclonal Antibody, Unconjugated(SL1461R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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