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Product Name phospho-EIF4B (Ser406) Chinese Name 磷酸化真核翻译起始因子4B抗体 Alias eIF4B (phospho S406); p-eIF4B (phospho S406); p-EIF4B (Ser406); p-EIF4B (S406); EIF 4B; EIF-4B; Eukaryotic initiation factor 4B; IF4B_HUMAN. Product Type Phosphorylated anti Research Area Transporter Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 69kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser406: HP(p-S)WR Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).
Function:
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Post-translational modifications:
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.
Similarity:
Contains 1 RRM (RNA recognition motif) domain.
SWISS:
P23588
Gene ID:
1975
Database links:Entrez Gene: 1975 Human
Entrez Gene: 75705 Mouse
Omim: 603928 Human
SwissProt: P23588 Human
SwissProt: Q8BGD9 Mouse
Unigene: 648394 Human
Unigene: 702041 Human
Unigene: 290022 Mouse
Unigene: 391795 Mouse
Unigene: 95954 Rat
Product Picture Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(SL14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD
Sample: Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(SL14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD
Sample: Hela Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-EIF4B (Ser406)(SL14551R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69kD
Observed band size: 69kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-EIF4B (Ser406)) Polyclonal Antibody, Unconjugated (SL14551R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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