Product Name	DNTTIP1
Chinese Name	末端脱氧核苷酸转移酶相互作用蛋白1抗体
Alias	Deoxynucleotidyltransferase terminal interacting protein 1; Deoxynucleotidyltransferase terminal-interacting protein 1; DNTTIP1; TdIF 1; TdIF1; TDIF1_HUMAN; TdT binding protein; TdT interacting factor 1; TdT-interacting factor 1; Terminal deoxynucleotidyltransferase interacting factor 1; Terminal deoxynucleotidyltransferase-interacting factor 1.
Research Area	Cell biology  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	37kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DNTTIP1: 21-120/329
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	DNTTIP1 binds DNA and enhances the activity of terminal deoxynucleotidyltransferase (TDT, or DNTT; MIM 187410), a DNA polymerase that catalyzes the polymerization of DNA in the absence of a DNA template (Yamashita et al., 2001 [PubMed 11473582]).[supplied by OMIM, Mar 2008] Subcellular Location: Nucleus. Tissue Specificity: Shown to enhance TdT activity, in vitro. SWISS: Q9H147 Gene ID: 116092 Database links: Entrez Gene: 116092 Human Omim: 611388 Human SwissProt: A6H7A8 Cow SwissProt: Q9H147 Human SwissProt: Q5R595 Orangutan Unigene: 472852 Human
Product Picture	Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-DNTTIP1 (SL14400R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 38 kD Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-DNTTIP1 (SL14400R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 38 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DNTTIP1) Polyclonal Antibody, Unconjugated (SL14400R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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