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Product Name DDX49 Chinese Name ATP依赖RNA解旋酶DDX49抗体 Alias DEAD (Asp Glu Ala Asp) box polypeptide 49; DEAD box protein 49; DDX49_HUMAN; FLJ10432; Probable ATP dependent RNA helicase DDX49; R27090 2. Research Area Cell biology Developmental biology transcriptional regulatory factor Binding protein Cell differentiation Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 45kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DDX49: 351-483/483 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis and cellular growth and division. DDX49 (DEAD-box protein 49) is a 483 amino acid protein that contains one helicase ATP-binding domain and one helicase C-terminal domain. One of several members of the DEAD-box protein family, DDX49 may function as a an RNA helicase that is involved in pre-mRNA splicing events.
Function:
The function of DDX49 has not yet been determined.
Similarity:
Belongs to the DEAD box helicase family. DDX49/DBP8 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
SWISS:
Q9Y6V7
Gene ID:
54555
Database links:Entrez Gene: 54555 Human
Entrez Gene: 234374 Mouse
SwissProt: Q9Y6V7 Human
SwissProt: Q3UTV0 Mouse
SwissProt: Q4FZF3 Mouse
Unigene: 143187 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDX49) Polyclonal Antibody, Unconjugated (SL14330R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDX49) Polyclonal Antibody, Unconjugated (SL14330R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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