Product Name	DHX37
Chinese Name	DHX37蛋白抗体
Alias	DDX37; DEAH (Asp-Glu-Ala-His) box polypeptide 37; DEAH box protein 37; FLJ41974; Gm1050; DHX37_HUMAN; Gm451; KIAA1517; MGC2695; MGC4322; MGC46245; MGC90028; mKIAA1517; Probable ATP dependent RNA helicase DHX37.
Research Area	Cell biology  Developmental biology  Cell differentiation  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	129kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DHX37: 51-150/1157
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a DEAD box protein. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. [provided by RefSeq, Jul 2008] Function: DHX37 is a member of the family of the DEAD box proteins, a group of proteins that present the conserved motif Asp-Glu-Ala-Asp (DEAD). They have a role in RNA translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly Similarity: Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: Q8IY37 Gene ID: 57647 Database links: Entrez Gene: 57647 Human Entrez Gene: 208144 Mouse SwissProt: Q8IY37 Human
Product Picture	Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-DHX37(SL14320R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 129kD Observed band size: 135kD Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DHX37) Polyclonal Antibody, Unconjugated (SL14320R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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