Product Name	DENND1A
Chinese Name	DENND1A蛋白抗体
Alias	Connecdenn 1; Connecdenn; DEN1A_HUMAN; DENN domain-containing protein 1A; DENN/MADD domain containing 1A; dennd1a; FAM31A; FLJ21129; FLJ38464; KIAA1608; OTTHUMP00000022090; Protein FAM31A; RP11 230L22.3.
Research Area	Cell biology  Neurobiology  Signal transduction  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	110kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DENND1A: 42-140/1009
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1A, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010] Function: Guanine nucleotide exchange factor (GEF) for RAB35. May be involved in the clathrin-mediated endocytosis of synaptic vesicles. Subcellular Location: Cell junction > synapse > presynaptic cell membrane. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Also associated with uncoated vesicles. Similarity: Contains 1 dDENN domain. Contains 1 DENN domain. Contains 1 uDENN domain. SWISS: Q8TEH3 Gene ID: 57706 Database links: Entrez Gene: 57706 Human Omim: 613633 Human SwissProt: Q8TEH3 Human Unigene: 568340 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DENND1A) Polyclonal Antibody, Unconjugated (SL14269R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DENND1A) Polyclonal Antibody, Unconjugated (SL14269R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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