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Product Name Deltex Chinese Name E3Ubiquitin蛋白连接酶抗体DTX1抗体 Alias Deltex homolog 1 (Drosophila); Deltex protein 1; Deltex-1; Deltex1; Dtx1; E3 ubiquitin protein ligase DTX1; FXI-T1; Fxit 1; Fxit1; DTX1_HUMAN; hDTX1; hDx 1; mDTX1; Protein deltex 1. Research Area Cell biology Neurobiology Transporter Epigenetics Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Pig, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 67kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Deltex: 351-450/620 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Studies in Drosophila have identified this gene as encoding a positive regulator of the Notch-signaling pathway. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity. [provided by RefSeq, Jul 2008]
Function:
Deltex is a RING finger ubiquitin ligase which is conserved from Drosophila to Humans and is a regulator of Notch signaling pathway. Deltex regulates both B-cell lineage and splenic marginal-zone B-cell commitment. Deltex is implicated in neurogenesis, lymphogenesis and myogenesis as well as marginal zone B cell differentiation.
Subunit:
Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1 via its N-terminus region and EIF3F, the interaction is required for NOTCH1 deubiquitination. Interacts with EP300. Forms a heterodimer with BBAP; the heterodimerization leading to an increase of in vitro ubiquitin ligase activity. Interacts with ITCH.
Subcellular Location:
Cytoplasm and Nucleus. Note: Predominantly cytoplasmic, partially nuclear.
Tissue Specificity:
Widely expressed. Strongly expressed in blood vessel. Also expressed in embryonic nervous system, pancreas, lung, adrenal gland, digestive tube and muscles. Expressed in MZB cells and developing B- and T-cells.
Post-translational modifications:
Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
Similarity:
Belongs to the Deltex family.
Contains 1 RING-type zinc finger.
Contains 2 WWE domains.
SWISS:
Q86Y01
Gene ID:
1840
Database links:Entrez Gene: 1840 Human
Entrez Gene: 14357 Mouse
Omim: 602582 Human
SwissProt: Q86Y01 Human
SwissProt: Q61010 Mouse
Product Picture Sample:
Kidney (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Deltex (SL14265R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67 kD
Observed band size: 67 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Deltex) Polyclonal Antibody, Unconjugated (SL14265R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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