Product Name	CYP8B1
Chinese Name	细胞色素P450 8B1抗体
Alias	7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; CP8B1_HUMAN; CYP12; CYP8B1; CYPVIIIB1; Cytochrome P450 8B1; Cytochrome P450, family 8, subfamily B, polypeptide 1; Sterol 12-alpha-hydroxylase.
literatures	Specific References  (1)     |     SL14165R has been referenced in 1 publications. [IF=17.694] Tang Bo. et al. Gut microbiota alters host bile acid metabolism to contribute to intrahepatic cholestasis of pregnancy. NAT COMMUN. 2023 Mar;14(1):1-17  IHC-P ;  Mouse.   PubMed:36894566
Research Area	Cell biology  Signal transduction  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	58kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CYP8B1: 151-250/501
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008] Function: Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids. Subcellular Location: Endoplasmic reticulum membrane. Microsome membrane. Tissue Specificity: Liver. Similarity: Belongs to the cytochrome P450 family. SWISS: Q9UNU6 Gene ID: 1582 Database links: Entrez Gene: 1582 Human Omim: 602172 Human SwissProt: Q9UNU6 Human Unigene: 447793 Human Unigene: 722743 Human
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-CYP8B1 (SL14165R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP8B1) Polyclonal Antibody, Unconjugated (SL14165R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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