Product Name	CRNKL1
Chinese Name	Apoptosis水解酶样蛋白1抗体
Alias	1200013P10Rik; 5730590A01Rik; C80326; CRN; Crn crooked neck like 1 (Drosophila); HCRN; MGC55327; MGC93283; MSTP021; SYF3; CRNL1_HUMAN; Crooked neck-like protein 1; Crooked neck homolog; hCrn.
Research Area	Cell biology  Developmental biology  Cyclin  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	100kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CRNKL1: 201-300/848
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013] Function: The crooked neck (crn) gene of Drosophila is essential for embryogenesis. It is thought to be involved in cell cycle progression and pre mRNA splicing. Subcellular Location: Nucleus; nuclear speckle. Cytoplasm. Colocalizes with core spliceosomal snRNP proteins. Also diffusely expressed in cytoplasm. Tissue Specificity: Widely expressed. Highly expressed in testis. Not expressed in brain and lung. Similarity: Belongs to the crooked-neck family. Contains 17 HAT repeats. SWISS: Q9BZJ0 Gene ID: 51340 Database links: Entrez Gene: 51340 Human Entrez Gene: 116481 Rat Omim: 610952 Human SwissProt: Q5JY65 Human SwissProt: Q9BZJ0 Human SwissProt: P63155 Rat Unigene: 171342 Human Unigene: 162694 Rat
Product Picture	Sample: Siha(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Primary: Anti- CRNKL1 (SL14065R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kD Observed band size: 98 kD Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CRNKL1 Polyclonal Antibody, Unconjugated(SL14065R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CRNKL1 Polyclonal Antibody, Unconjugated(SL14065R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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