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Product Name phospho-Cortactin (Tyr421) Chinese Name 磷酸化皮层肌动蛋白抗体 Alias Cortactin (phospho Y421); p-Cortactin (phospho Y421); Amplaxin; CTTN; EMS 1; EMS1; FLJ34459; Mammary tumor and squamous cell carcinoma associated; Oncogene EMS1; p80/85 src substrate; Src substrate cortactin; SRC8_HUMAN. Product Type Phosphorylated anti Research Area Tumour Cell biology Signal transduction Apoptosis Kinases and Phosphatases Cytoskeleton Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 62kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human Cortactin around the phosphorylation site of Tyr421: PV(p-Y)ED Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]
Function:
Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
Subcellular Location:
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
Post-translational modifications:
Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
Similarity:
Contains 7 cortactin repeats.
Contains 1 SH3 domain.
SWISS:
Q14247
Gene ID:
2017
Database links:Entrez Gene: 2017 Human
Entrez Gene: 13043 Mouse
GenBank: NM_138565 Human
GenBank: NM_007803 Mouse
Omim: 164765 Human
SwissProt: Q14247 Human
SwissProt: Q60598 Mouse
Unigene: 596164 Human
Unigene: 205601 Mouse
Unigene: 107869 Rat
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