Product Name	phospho-COP1 (Ser387)
Chinese Name	磷酸化E3Ubiquitin蛋白连接酶COP1抗体
Alias	COP1 (phospho S387); p-COP1 (phospho S387); Constitutive photomorphogenesis protein 1 homolog; COP; E3 ubiquitin-protein ligase RFWD2; hCOP1; RFWD2; RFWD2_HUMAN; RNF200; ring finger and WD repeat domain 2; RING finger and WD repeat domain protein 2; RING finger protein 200.
Product Type	Phosphorylated anti
Research Area	Signal transduction  Epigenetics  Ubiquitin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	80kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human COP1 around the phosphorylation site of Ser387: TA(p-S)QL
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	COP1 is an E3 ubiquitin ligase protein that mediates ubiquitination and degradation of target proteins such as c-Jun and p53. It is a component of the DCX DET1-COP1 ubiquitin ligase complex which consists of RBX1, DET1, DDB1, CUL4A and COP1. Localizing to the cytoplasm and to the nucleus, COP1 is primarily expressed in testis, placenta, heart and skeletal muscle. COP1 is a potent inhibitor of p53-dependent transcription and apoptosis but, when phosphorylated by Atm (ataxia telangiectasia mutated) in response to DNA damage, the COP1-p53 complex is disrupted and p53 is allowed to exert its pro-apoptotic properties. In ovarian and breast cancers, COP1 is overexpressed, suggesting a role for COP1 in tumorigenesis. Function: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Subcellular Location: Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles. Tissue Specificity: Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. Similarity: Belongs to the COP1 family. Contains 1 RING-type zinc finger. Contains 7 WD repeats. SWISS: Q8NHY2 Gene ID: 64326 Database links: Entrez Gene: 64326 Human Entrez Gene: 26374 Mouse Omim: 608067 Human SwissProt: Q8NHY2 Human SwissProt: Q9R1A8 Mouse Unigene: 523744 Human Unigene: 328135 Mouse
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-COP1 (Ser387)) Polyclonal Antibody, Unconjugated (SL13976R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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