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Product Name HSP105 Chinese Name 热休克蛋白105抗体 Alias Antigen NY CO 25; Antigen NY-CO-25; heat shock 105kDa protein; heat shock 105kDa/110kDa protein 1; heat shock 110kDa protein; HS105_HUMAN; Heat shock 105kD alpha; Heat shock 105kD; Heat shock 105kD beta; Heat shock 105kDa protein 1; Heat shock 105kDa protein; Heat shock 105kDa/110kDa protein 1; Heat shock 110kDa protein; Heat shock protein 105 kDa; HSP105; HSP105A; HSP105B; HSP110; HSPH 1; HSPH1; KIAA0201; NY CO 25; Antigen NY CO 25; DKFZp686M05240. Research Area immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 97kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human HSP105: 201-350/858 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced when a cell undergoes various types of environmental stresses like heat, cold and oxygen deprivation. They are characterized by their role as molecular chaperones.
Heat shock protein (HSP) 105 exists as two isoforms; alpha and beta which belong to the HSP105/HSP110 protein family. HSP105 acts as both a chaperone to prevent thermal aggregation of proteins and as a regulator of mammalian cells. The HSP105 isoforms are found in the cytoplasm but not in the nucleoli under non-stressed and stressed conditions.
Function:
Prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly. Inhibits HSPA8/HSC70 ATPase and chaperone activities (By similarity).
Subunit:
Interacts with HSPA8/HSC70.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Highly expressed in testis. Present at lower levels in most brain regions, except cerebellum. Overexpressed in cancer cells.
Post-translational modifications:
Phosphorylation on Ser-509 may be important for regulation of the HSPA8/HSC70 chaperone activity.
Similarity:
Belongs to the heat shock protein 70 family.
SWISS:
Q92598
Gene ID:
10808
Database links:Entrez Gene: 10808 Human
Entrez Gene: 15505 Mouse
Omim: 610703 Human
SwissProt: Q92598 Human
SwissProt: Q61699 Mouse
Unigene: 36927 Human
Unigene: 270681 Mouse
Unigene: 37805 Rat
Product Picture Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSP105 Polyclonal Antibody, Unconjugated(SL1387R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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