Product Name	CES4A
Chinese Name	羧酸酯酶4A抗体
Alias	Carboxylesterase 4A; Carboxylesterase 6; Carboxylesterase 8 (putative); Ces4a; CES6; CES8; EC 3.1.1.-; EST4A_HUMAN; FLJ37002; FLJ37464; OTTHUMP00000237118; OTTHUMP00000237120; OTTHUMP00000237122; OTTHUMP00000237123.
Research Area	Cell biology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	61kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CES4A: 491-561/561
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They also participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene, also called CES6, encodes a secreted enzyme, and may play a role in the detoxification of drugs and xenobiotics in neural and other tissues of the body and in the cerebrospinal fluid. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature and/or biological validity of some variants have not been determined. [provided by RefSeq, Jun 2010] Function: Probable carboxylesterase. Subcellular Location: Secreted. Similarity: Belongs to the type-B carboxylesterase/lipase family. SWISS: Q5XG92 Gene ID: 283848 Database links: Entrez Gene: 283848 Human SwissProt: Q5XG92 Human Unigene: 346947 Human
Product Picture	Sample: Brain (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-CES4A (SL13870R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 50 kD Tissue/cell: human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CES4A Polyclonal Antibody, Unconjugated(SL13870R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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