Product Name	Centaurin alpha 2
Chinese Name	CENTA2蛋白抗体
Alias	ADAP2; ADAP2_HUMAN; Arf-GAP with dual PH domain-containing protein 2; ArfGAP with dual PH domains 2; cent b; Centa 2; CENTA2; Centaurin alpha 2; Centaurin alpha 2 protein; Centaurin beta; Centaurin-alpha-2; Cnt-a2; HSA272195.
Research Area	Cardiovascular  Cell biology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	44kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Centaurin alpha 2: 201-300/381
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The ADP-ribosylation factor (ARF) family of small GTP-binding proteins are involved in vesicular transport regulation and in controlling cytoskeletal organization and cell adhesion. The Centaurin GTPase-activating protein family comprise a subset of ARF regulatory molecules that transduce PI 3-kinase activation into coordinated control of ARF-dependent pathways. This family includes ASAP1, ACAP1, ACAP2, AGAP1, ARAP1, ARAP2, Centaurin a1, Centaurin g3 and the recently discovered Centaurin a2. Expressed in a wide variety of tissues such as fat, heart and skeletal muscle, Centaurin a2 is thought to negatively regulate ARF-mediated actin rearrangement by binding activated PI 3-kinase. Although the exact function of Centaurin a2 is not yet known, its high sequence similarity with Centaurin a1 suggests that it may also act as an ARF6 GTPase. Function: GTPase-activating protein for the ADP ribosylation factor family (Potential). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). Possesses a stoichiometry of two binding sites for InsP4 with identical affinity. Subcellular Location: Cytoplasm. Cell membrane. Constitutively associated with the plasma membrane. Excluded from the nucleus. Tissue Specificity: Highly expressed in placenta, spleen, kidney, skeletal muscle and adrenal gland. Weakly expressed in thyroid, liver, heart, lung, small intestine, peripheral blood leukocytes. Not detected in spinal cord, brain, stomach, trachea, colon, lymph node and bone marrow. Similarity: Contains 1 Arf-GAP domain. Contains 2 PH domains. SWISS: Q9NPF8 Gene ID: 55803 Database links: Entrez Gene: 55803 Human Entrez Gene: 216991 Mouse Entrez Gene: 56826 Rat SwissProt: Q9NPF8 Human SwissProt: Q8R2V5 Mouse SwissProt: Q9JK15 Rat Unigene: 514063 Human Unigene: 341520 Mouse Unigene: 203877 Rat
Product Picture	Sample: MCF-7 Cell (Human) Lysate at 30 ug Primary: Anti-Centaurin alpha 2 (SL13841R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44kD Observed band size: 44kD Paraformaldehyde-fixed, paraffin embedded (Rat adrenal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Centaurin alpha 2) Polyclonal Antibody, Unconjugated (SL13841R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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