Product Name	MAP2
Chinese Name	微管相关蛋白2抗体
Alias	Microtubule-associated protein 2; DKFZp686I2148; Dendrite specific MAP; DKFZp686I2148; MAP 2; MAP-2; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Mtap 2; MAP2_HUMAN.
literatures	Specific References  (23)     |     SL1369R has been referenced in 23 publications. [IF=13.281] Yu Hei. et al. Multifunctional Immunoliposomes Enhance the Immunotherapeutic Effects of PD-L1 Antibodies against Melanoma by Reprogramming Immunosuppressive Tumor Microenvironment. 2021 Dec 16  IF,IHC ;  Mouse.   PubMed:34915595 [IF=11.092] Yan Hu. et al. An ECM-Mimicking, Injectable, Viscoelastic Hydrogel for Treatment of Brain Lesions. ADV HEALTHC MATER. 2022 Nov;:2201594  IF ;  Mouse.   PubMed:36398536 [IF=6.291] Hongmei Zhou. et al. Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain. Ecotox Environ Safe. 2022 Jan;229:113066  IF ;  Mouse.   PubMed:34929507 [IF=5.878] Lin Zhu. et al. Neuroprotective effects of salidroside on ageing hippocampal neurons and naturally ageing mice via the PI3K/Akt/TERT pathway. 2021 Aug 09  WB ;  Rat.   PubMed:34374127 [IF=5.753] Chaohua Cong. et al. PAK4 suppresses motor neuron degeneration in hSOD1G93A‐linked amyotrophic lateral sclerosis cell and rat models. 2021 Feb 21  IF,IHC ;  Mouse.   PubMed:33615605 [IF=5.75] Zhifei Wang. et al. Human Cytomegalovirus Immediate Early Protein 2 Protein Causes Cognitive Disorder by Damaging Synaptic Plasticity in Human Cytomegalovirus-UL122-Tg Mice. Front Aging Neurosci. 2021; 13: 720582  IF,IHC ;  Mouse.   PubMed:34790111 [IF=5.391] Jiang Y et al. A mutation in MAP2 is associated with prenatal hair follicle density. ASEB J. 2019 Dec;33(12):14479-14490.  IHC-P ;  Pig.   PubMed:31751154 [IF=5.295] Xiahong Tang. et al. Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 May;:  IF,ICC ;  Rat.   PubMed:37246833 [IF=5.16] Yang YQ et al. Wild-type p53-induced phosphatase 1 down-regulation promotes apoptosis by activating the DNA damage-response pathway in amyotrophic lateral sclerosis. Neurobiol Dis. 2019 Oct 30;134:104648.  IHF ;  Mouse.   PubMed:31676238 [IF=4.571] Lingbing Zhang. et al. Disruption of the lung-gut-brain axis is responsible for cortex damage induced by pulmonary exposure to zinc oxide nanoparticles. TOXICOLOGY. 2022 Dec;:153390  IF ;  Mouse.   PubMed:36535435 [IF=4.35] Gu, LiZe, et al. "Early activation of nSMase2/ceramide pathway in astrocytes is involved in ischemia-associated neuronal damage via inflammation in rat hippocampi." Journal of Neuroinflammation 10.1 (2013): 109.  Rat.   PubMed:24007266 [IF=3.97] Lu, Lihua, et al. "AMP-activated protein kinase activation in mediating phenylalanine-induced neurotoxicity in experimental models of phenylketonuria." Journal of inherited metabolic disease (2017): 1-9.  WB ;  Mouse.   PubMed:29230603 [IF=3.414] Wang K et al. Harpagide from Scrophularia protects rat cortical neurons from oxygen-glucose deprivation and reoxygenation-induced injury by decreasing endoplasmic reticulum stress. J Ethnopharmacol. 2020 Jan 31;253:112614.  WB ;  Rat.   PubMed:32007630 [IF=3.265] Zhang Y et al. Exposure to carbon black nanoparticles during pregnancy persistently damages the cerebrovascular function in female mice. Toxicology. 2019 Apr 22;422:44-52.  IHF&WB ;  Mouse.   PubMed:31022427 [IF=3.241] Che H et al. EPA-Enriched Ethanolamine Plasmalogen and EPA-Enriched Phosphatidylethanolamine Enhance BDNF/TrkB/CREB Signaling and inhibit Neuronal Apoptosis in vitro and in vivo. Food Funct. 2020 Feb 11.  ICF ;  Rat.   PubMed:32043504 [IF=3.105] Shanshan Zhang. et al. Pregnancy exposure to carbon black nanoparticles induced neurobehavioral deficits that are associated with altered m6A modification in offspring. Neurotoxicology. 2020 Dec;81:40  WB ;  Mouse.   PubMed:32783905 [IF=3.076] Tang Q et al. Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure.Neurotoxicology. 2018 Jul;67:27-36.  IF ;  Mouse.   PubMed:29678591 [IF=2.772] Zhou J et al. Imbalance of Microglial TLR4/TREM2 in LPS-Treated APP/PS1 Transgenic Mice: A Potential Link Between Alzheimer's Disease and Systemic Inflammation Neurochemical Research.2019.  TUNEL ;  Mouse.   PubMed:10.1007/s11064-019-02748-x [IF=2.65] Zuo, Daiying, et al. "Existence of glia mitigated ketamine-induced neurotoxicity in neuron-glia mixed cultures of neonatal rat cortex and the glia-mediated protective effect of 2-PMPA." Neurotoxicology (2014).  Rat.   PubMed:24931484 [IF=2.3] Wang, Jin, et al. "Neuroprotective Effects of Wnt/ß-catenin signaling pathway against Aβ-induced Tau protein over-phosphorylation in PC12 cells."Biochemical and Biophysical Research Communications (2016).  Rat.   PubMed:26809093 [IF=2.276] Zhang Heng. et al. Bone Morphogenetic Protein-7 (BMP-7) Promotes Neuronal Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) In Vitro. Biomed Res Int. 2021;2021:7239783  WB,IF,ICC ;  Rat.   PubMed:33575343 [IF=1.61] Usui, Tatsuya, et al. "Establishment of a novel three‐dimensional primary culture model for hippocampal neurogenesis." Physiological Reports 5.12 (2017): e13318.  IF(IHC-P) ;  Mouse.   PubMed:28642339 [IF=1.18] Ma Caiyun. et al. In Vitro Culture and Multipotency Evaluation of Broiler Umbilical Cord Mesenchymal Stem Cells. BRAZ ARCH BIOL TECHN. 2023 Feb;66:  IF ;  Human.   PubMed:10.1590/1678-4324-2023220031
Research Area	Tumour  Cell biology  immunology  Neurobiology  Signal transduction  Apoptosis  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:200-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	70/201kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MAP2: 1-120/1827
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton. Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Subcellular Location: Cytoplasm, cytoskeleton (Probable). Post-translational modifications: Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. Isoform MAP2c is phosphorylated by FYN at Tyr-67. Similarity: Contains 3 Tau/MAP repeats. SWISS: P11137 Gene ID: 4133 Database links: Entrez Gene: 4133 Human Entrez Gene: 25595 Rat Omim: 157130 Human SwissProt: P11137 Human SwissProt: P20357 Mouse SwissProt: P15146 Rat Unigene: 368281 Human Unigene: 256966 Mouse 微管相关蛋白 2（MAP-2)是一种磷蛋白质，在正常脑组织中，主要存在于神经元的胞体、树突和树突棘，是脑内最丰富的蛋白之一。MAP-2在其调节微管的聚合作用和微管的稳定性以及对神经元轴突和树突的发生、延长、稳定和突触可塑性调节具有重要作用。
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-MAP2 (SL1369R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 201 kD Observed band size: 280 kD Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP2) Polyclonal Antibody, Unconjugated (SL1369R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP2) Polyclonal Antibody, Unconjugated (SL1369R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP2) Polyclonal Antibody, Unconjugated (SL1369R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAP2) Polyclonal Antibody, Unconjugated (SL1369R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated(SL1369R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated (SL0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-MAP2 antibody (SL1369R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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