Product Name	DIP13B
Chinese Name	DIP13β抗体
Alias	Adapter protein containing PH domain; Adapter protein containing PH domain PTB domain and leucine zipper motif 2; adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2; APLP2; Appl2; DCC interacting protein 13 beta; DCC-interacting protein 13-beta; Dip13 beta; Dip13-beta; DP13B_HUMAN; PTB domain and leucine zipper motif 2.
Research Area	Tumour  Cell biology  Signal transduction  The cell membrane受体  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Chicken, Dog, Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	74kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DIP13B/APPL2: 101-200/664
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The APPL family of proteins are involved in linking, trafficking and signaling downstream of tyrosine kinase receptors. APPL1, also designated adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; APPL; or DCC interacting protein 13å (DIP13å), and APPL2, also designated adaptor protein containing pH domain, PTB domain and leucine zipper motif 2 or DCC interacting protein 13∫ (DIP13∫), are involved in the coupling of epidermal growth factor (EGF) signaling and chromatin remodeling in the nucleus. They associate with GTPase Rab 5 and are released from the plasma membrane and translocated to the nucleus. In the nucleus, APPL1 and APPL2 associate with NuRD/MeCP1 and are essential for cell growth and proliferation. APPL2 also associates with follicle stimulating hormone receptor (FSHR). APPL2 is highly expressed in heart, brain, skeletal muscle, and kidney. APPL2 shares 54% homology with APPL1 Function: Required for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction. Subcellular Location: Early endosome membrane. Nucleus. Early endosomal membrane-bound and nuclear. Translocated into the nucleus upon release from endosomal membranes following internalization of EGF. Tissue Specificity: High levels in brain, heart, kidney and skeletal muscle. DISEASE: Note=A chromosomal aberration involving APPL2/DIP13B is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with SHANK3/PSAP2. Similarity: Contains 1 PH domain. Contains 1 PID domain. SWISS: Q8NEU8 Gene ID: 55198 Database links: Entrez Gene: 55198 Human Entrez Gene: 216190 Mouse Entrez Gene: 362860 Rat Omim: 606231 Human SwissProt: Q8NEU8 Human SwissProt: Q8K3G9 Mouse Unigene: 506603 Human Unigene: 282985 Mouse
Product Picture	Sample:Lovo Cell (Human) Lysate at 30 ug Primary: Anti-DIP13B (SL13631R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74kD Observed band size: 76kD Sample:293T Cell (Human) Lysate at 30 ug Primary: Anti-DIP13B (SL13631R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74kD Observed band size: 76kD Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DCC interacting protein 13 beta; DIP13B) Polyclonal Antibody, Unconjugated (SL13631R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes .

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