Product Name	CD53
Chinese Name	CD53抗体
Alias	AI323659; Antigen MOX44 identified by monoclonal; MRC-OX44; CD 53; CD53 antigen; CD53 antigen, isoform CRA_b; CD53 glycoprotein; Cd53 molecule; CD53 tetraspan antigen; CD53_HUMAN; Cell surface glycoprotein CD53; Leukocyte antigen MRC OX-44; Leukocyte surface antigen CD53; MOX 44; MOX44; Ox-44; OX44; RATOX44; Tetraspanin 25; Tetraspanin-25; Tetraspanin25; Transmembrane glycoprotein; TSPAN 25; Tspan-25; TSPAN25.
Research Area	Tumour  Cell biology  Signal transduction  Cell adhesion molecule  Cell Surface Molecule  glycoprotein  lymphocyte  t-lymphocyte  b-lymphocyte
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	24kDa
Detection molecular weight	35-45kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD53: 65-160/219 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] Function: Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity). Subunit: Interacts with SCIMP. Subcellular Location: Membrane. Tissue Specificity: B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells. Similarity: Belongs to the tetraspanin (TM4SF) family. SWISS: P19397 Gene ID: 963 Database links: Entrez Gene: 963 Human Omim: 151525 Human SwissProt: P19397 Human Unigene: 443057 Human
Product Picture	Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti- CD53 (SL13625R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 25 kD Sample: Molt-4(Human) Cell Lysate at 30 ug Primary: Anti- CD53 (SL13625R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 26 kD Sample: Jurkat(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug Primary: Anti- CD53 (SL13625R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 24 kD Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD53 Polyclonal Antibody, Unconjugated(SL13625R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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