Product Name	TMEM176A
Chinese Name	Transmembrane protein176A抗体
Alias	GS188; HCA112; Hepatocellular carcinoma-associated antigen 112; likley ortholog of mouse GS188; T176A_MOUSE; Tmem176a; Transmembrane protein 176A.
Research Area	Tumour  Cell biology  Neurobiology  Stem cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	26kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from MOUSE TMEM176A: 1-100/235
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	TMEM176A is a 235 amino acid multi-pass membrane protein belonging to the TMEM176 family. The gene encoding GS188 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Chromosome 7 has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. The deletion of a portion of the q arm of chromosome 7 is associated with Williams-Beuren syndrome, a condition characterized by mild mental retardation, an unusual comfort and friendliness with strangers and an elfin appearance. Deletions of portions of the q arm of chromosome 7 are also seen in a number of myeloid disorders including cases of acute myelogenous leukemia and myelodysplasia. Subcellular Location: Membrane. Similarity: Belongs to the TMEM176 family. SWISS: Q96HP8 Gene ID: 55365 Database links: Entrez Gene: 55365 Human Omim: 610334 Human SwissProt: Q96HP8 Human Unigene: 647116 Human
Product Picture	Protein: HepG2(human) lysates at 30ug; Primary: rabbit Anti-TMEM176A (SL13622R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size:26 kD Observed band size:26 kD Sample: U937(Human) Cell Lysate at 30 ug Primary: Anti- TMEM176A (SL13622R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 25 kD Protein: mouse kidney lysates, 30ug; Primary: Anti-TMEM176A (SL13622R) at 1:300; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1:5000; ECL excitated the fluorescence; Predicted band size : 24kD Observed band size : 24kD Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TMEM176A Polyclonal Antibody, Unconjugated(SL13622R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM176A) Polyclonal Antibody, Unconjugated (SL13622R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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