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Product Name GNL2 Chinese Name 鸟嘌呤核苷酸Binding protein2抗体 Alias Autoantigen NGP-1; Autoantigen NGP1; DJ423B22.6 (novel nucleolar guanosine 5'-triphosphate binding protein; FLJ40906; GNL2; Guanine nucleotide binding protein-like 2 (nucleolar); HUMAUANTIG; NGP1; NOG2_HUMAN; Nucleolar GTP binding protein 2; Nucleolar GTP-binding protein 2; Nucleolar GTPase. Research Area Cell biology Signal transduction G protein-coupled receptor Epigenetics G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 84kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GNL2: 1-100/731 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail GNL2 is a nucleolar guanasine-triphosphate binding protein that is ubiquitously expressed at low levels in almost all tissues. GNL2 is involved in the crucial process of trafficking proteins out of the nucleus. Specifically, it is a GTPase that interacts with the 60s preribosomal subunit in the nucleus and facilitates export of the subunit into the cytoplasm. GTPases are responsible for the hydrolysis of GTP by way of a protein region dubbed the G domain. GTPases are often involved in the translocating proteins through membranes gleaning energy for the activity by hydrolizing GTP. GNL2 shares G domain homology and some functionality with nucleostemin (GNL3), another nuclear GTPase. Highest expression of GNL2 is found in testis.
Function:
GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.
Subcellular Location:
Nucleus; nucleolus.
Tissue Specificity:
Ubiquitously expressed at relatively low levels in all human tissues tested, with the highest level of expression in the testes.
Similarity:
Belongs to the MMR1/HSR1 GTP-binding protein family. NOG2 subfamily.
Contains 1 G (guanine nucleotide-binding) domain.
SWISS:
Q13823
Gene ID:
29889
Database links:Entrez Gene: 29889 Human
Entrez Gene: 230737 Mouse
Omim: 609365 Human
SwissProt: Q13823 Human
SwissProt: Q99LH1 Mouse
Unigene: 75528 Human
Unigene: 90760 Mouse
Unigene: 18964 Rat
Product Picture Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- GNL2 (SL13471R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GNL2 Polyclonal Antibody, Unconjugated(SL13471R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GNL2 Polyclonal Antibody, Unconjugated(SL13471R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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