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Product Name GLYR1 Chinese Name 氧化还原酶GlyR1/核蛋白60抗体 Alias 3 hydroxyisobutyrate dehydrogenase like protein; 3-hydroxyisobutyrate dehydrogenase-like protein; BM045; Cytokine like nuclear factor n pac; Cytokine-like nuclear factor N-PAC; Glyoxylate reductase 1 homolog; GLYR1; GLYR1_HUMAN; HIBDL; N-PAC; NP60; Nuclear protein 60 kDa; Nuclear protein NP60; Nuclear protein of 60 kDa; Putative oxidoreductase GLYR1. Research Area Cell biology Signal transduction Kinases and Phosphatases Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Monkey, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 61kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GLYR1/NP60: 21-120/553 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail NP60 is a 553 amino acid nuclear protein that regulates the phosphorylation and activation of p38 alpha in response to stress. There are five isoforms of NP60 that are produced as a result of alternative splicing events.
Function:
May have oxidoreductase activity. Regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 signaling. Indirectly promotes phosphorylation of MAPK14 and activation of ATF2. The phosphorylation of MAPK14 requires upstream activity of MAP2K4 and MAP2K6. Recruited on chromatin, recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3).
Subunit:
Interacts with MAPK14.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the 3-hydroxyisobutyrate dehydrogenase family.
NP60 subfamily. Contains 1 A.T hook DNA-binding domain.
Contains 1 PWWP domain.
SWISS:
Q49A26
Gene ID:
84656
Database links:Entrez Gene: 84656 Human
Entrez Gene: 74022 Mouse
Omim: 610660 Human
SwissProt: Q49A26 Human
SwissProt: Q922P9 Mouse
Unigene: 387255 Human
Unigene: 21652 Mouse
Unigene: 2639 Rat
Product Picture Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- GLYR1 (SL13451R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Stomach (Mouse) Lysate at 40 ug
Liver (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- GLYR1 (SL13451R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
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