TEL: +86 571 56623320 EMAIL: [email protected]
Product Name QARS/Glutamyl Prolyl tRNA synthetase/ProRS Chinese Name 谷氨酰-脯氨酰-tRNA合成酶抗体 Alias Bifunctional aminoacyl tRNA synthetase; Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline tRNA ligase; Cell proliferation-inducing gene 32 protein; DKFZp313B047; EARS; Eprs; GLNS; Glu pro tRNA synthetase; GLUPRORS; GluRS; Glutamate tRNA ligase; Glutamatyl prolyl tRNA synthetase; Glutaminyl tRNA synthetase; Glutamyl prolyl tRNA synthetase; Glutamyl tRNA synthetase; Glutamyl-tRNA synthetase; PARS; PIG 32; PIG32; Proliferation inducing gene 32 protein; Proliferation inducing protein 32; Proline tRNA ligase; Proline--tRNA ligase; Prolyl tRNA synthetase; Prolyl-tRNA synthetase; QARS; QPRS; SYEP_HUMAN. Research Area Cell biology Signal transduction Transporter Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 171kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human ProRS: 651-750/1512 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. ProRS (Prolyl-tRNA synthetase), also known as EPRS, EARS, PARS, QARS, QPRS, PIG32 or GLUPRORS, is a 1,512 amino acid protein that contains three WHEP-TRS domains and belongs to both the class-I and class-II aminoacyl-tRNA synthetase family. Functioning as a component of the multisynthase complex, ProRS uses ATP to catalyze the conversion of L-glutamate and tRNA(Glu) to L-glutamyl-tRNA(Glu), as well as the conversion of L-proline and tRNA(Pro) to L-prolyl-tRNA(Pro).
Function:
Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.
Subunit:
Component of the multisynthetase complex which iscomprised of a bifunctional glutamyl-prolyl-tRNA synthetase, themonospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl,arginyl, and aspartyl-tRNA synthetases as well as three auxiliaryproteins, p18, p48 and p43. Interacts with DUS2L. Component of theGAIT complex; in humans the complex assembly seems to be a two-stepprocess in which EPRS first associates with SYNCRIP to form apre-GAIT complex which is deficient in GAIT element binding.
Post-translational modifications:
Phosphorylated at Ser-886 by CDK5 in a IFN-gamma-dependent manner; this phosphorylation activates assembly of the GAIT (IFN-gamma-activated inhibitor of translation) complex, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.
Similarity:
In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.
Contains 3 WHEP-TRS domains.
SWISS:
P07814
Gene ID:
5859
Database links:Entrez Gene: 5859 Human
Omim: 603727 Human
SwissProt: P47897 Human
Unigene: 79322 Human
Product Picture Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti- QARS (SL13393R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 171 kD
Observed band size: 171 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (SL13393R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (SL13393R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (QARS Glutamyl Prolyl tRNA synthetase) Polyclonal Antibody, Unconjugated (SL13393R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Glutamyl Prolyl tRNA synthetase/ProRS Polyclonal Antibody, Unconjugated(SL13393R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Scan Wechat Qrcode