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Rabbit Anti-GLT28D1 antibody
Rabbit Anti-GLT28D1 antibody
ALG13; GLT28D1; ALG13_HUMAN; Asparagine linked glycosylation 13 homolog (S. cerevisiae); Asparagine-linked glycosylation 13 homolog; CXorf45; FLJ23018; FLJ31785; GLT28D1; Glycosyltransferase 28 domain containing protein 1; Glycosyltransferase 28 domain-co
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  • NO.:SL13380R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Rat,(predicted: Human,)
    Applications:WB ELISA IHC-P IHC-F ICC IF
    concentration:1mg/ml
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Details

Product Name GLT28D1
Chinese Name 糖基转移酶28家族1抗体
Alias ALG13; GLT28D1; ALG13_HUMAN; Asparagine linked glycosylation 13 homolog (S. cerevisiae); Asparagine-linked glycosylation 13 homolog; CXorf45; FLJ23018; FLJ31785; GLT28D1; Glycosyltransferase 28 domain containing protein 1; Glycosyltransferase 28 domain-containing protein 1; Hematopoietic stem/progenitor cells protein MDS031; MDS031; MGC12423; UDP N acetylglucosamine transferase subunit ALG13 homolog; UDP-N-acetylglucosamine transferase subunit ALG13 homolog; YGL047W.  
Research Area Cell biology  Signal transduction  Cell type markers  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Rat,  (predicted: Human, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 126kDa
Cellular localization cytoplasmic The cell membrane 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human GLT28D1/ALG13: 21-120/1137 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail ALG13 is a 1,137 amino acid protein belonging to the glycosyltransferase 28 family. Encoded by a gene that maps to human chromosome Xq23, ALG13 is a subunit of a bipartite UDP-N-acetylglucosamine transferase and plays a role in protein folding regulation and stabilization. ALG13 contains one OTU domain, one TudorSN domain, and exists as four alternatively spliced isoforms. Heterodimerizing with ALG14, ALG13 forms a UDP-GlcNAc glycosyltransferase, which catalyzes the second sugar addition of the oligosaccharide precursor in endoplasmic reticulum (ER) N-linked glycosylation. ALG13 localizes to ER and may be recruited to the cytosolic face of the membrane by interacting with ALG14.

Function:
Isoform 2 may be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway.

Subunit:
Isoform 2 may interact with ALG14.

Subcellular Location:
Endoplasmic reticulum. Could be recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14.

Similarity:
Belongs to the glycosyltransferase 28 family.
Contains 1 OTU domain.
Contains 1 Tudor domain.

SWISS:
Q9NP73

Gene ID:
79868

Database links:

Entrez Gene: 79868 Human

Omim: 300776 Human

SwissProt: Q9NP73 Human

Unigene: 443061 Human



Product Picture
Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLT28D1 Polyclonal Antibody, Unconjugated(SL13380R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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