TEL: +86 571 56623320 EMAIL: [email protected]
Product Name GLT28D1 Chinese Name 糖基转移酶28家族1抗体 Alias ALG13; GLT28D1; ALG13_HUMAN; Asparagine linked glycosylation 13 homolog (S. cerevisiae); Asparagine-linked glycosylation 13 homolog; CXorf45; FLJ23018; FLJ31785; GLT28D1; Glycosyltransferase 28 domain containing protein 1; Glycosyltransferase 28 domain-containing protein 1; Hematopoietic stem/progenitor cells protein MDS031; MDS031; MGC12423; UDP N acetylglucosamine transferase subunit ALG13 homolog; UDP-N-acetylglucosamine transferase subunit ALG13 homolog; YGL047W. Research Area Cell biology Signal transduction Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 126kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GLT28D1/ALG13: 21-120/1137 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ALG13 is a 1,137 amino acid protein belonging to the glycosyltransferase 28 family. Encoded by a gene that maps to human chromosome Xq23, ALG13 is a subunit of a bipartite UDP-N-acetylglucosamine transferase and plays a role in protein folding regulation and stabilization. ALG13 contains one OTU domain, one TudorSN domain, and exists as four alternatively spliced isoforms. Heterodimerizing with ALG14, ALG13 forms a UDP-GlcNAc glycosyltransferase, which catalyzes the second sugar addition of the oligosaccharide precursor in endoplasmic reticulum (ER) N-linked glycosylation. ALG13 localizes to ER and may be recruited to the cytosolic face of the membrane by interacting with ALG14.
Function:
Isoform 2 may be involved in protein N-glycosylation, second step of the dolichol-linked oligosaccharide pathway.
Subunit:
Isoform 2 may interact with ALG14.
Subcellular Location:
Endoplasmic reticulum. Could be recruited to the cytosolic face of the endoplasmic reticulum membrane through its interaction with ALG14.
Similarity:
Belongs to the glycosyltransferase 28 family.
Contains 1 OTU domain.
Contains 1 Tudor domain.
SWISS:
Q9NP73
Gene ID:
79868
Database links:Entrez Gene: 79868 Human
Omim: 300776 Human
SwissProt: Q9NP73 Human
Unigene: 443061 Human
Product Picture Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLT28D1 Polyclonal Antibody, Unconjugated(SL13380R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Scan Wechat Qrcode