Product Name	MBOAT4
Chinese Name	脑肠肽O酰基转移酶/Ghrelin O acyltransferase抗体
Alias	FKSG89; ghrelin o transferas; Ghrelin O-acyltransferase; GOAT; MBOA4_HUMAN; Mboat4; Membrane-bound O-acyltransferase domain-containing protein 4; O-acyltransferase (membrane bound) domain containing 4; O-acyltransferase domain-containing protein 4; OACT4.
literatures	Specific References  (3)     |     SL13355R has been referenced in 3 publications. [IF=3.707] Yuan Zhang. et al. The Ghrelin/Growth Hormone Secretagogue Receptor System Is Involved in the Rapid and Sustained Antidepressant-Like Effect of Paeoniflorin. Front Neurosci-Switz. 2021; 15: 631424  WB ;  Mouse.   PubMed:33664648 [IF=3.27] Yu, Ching‐Han, et al. "Mediation of oxidative stress in hypothalamic ghrelin‐associated appetite control in rats treated with phenylpropanolamine." Genes, Brain and Behavior (2016).  WB ;  Rat.   PubMed:27862969 [IF=3.12] Yu, Ching-Han, et al. "Participation of ghrelin signalling in the reciprocal regulation of hypothalamic NPY/POMC-mediated appetite control in amphetamine-treated rats." Appetite (2017).  WB ;  Rat.   PubMed:28229938
Research Area	Cell biology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Horse, Rabbit, Monkey, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Ghrelin O acyltransferase: 101-200/435
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	MBOAT4 (membrane-bound O-acyltransferase domain-containing protein 4, Ghrelin O-acyltransferase) is a 435 amino acid, multi-pass membrane protein that belongs to the membrane-bound acyltransferase family. MBOAT4 functions as an enzyme that attaches an octanoate fatty acid to serine-3 of ghrelin. Ghrelin is a very small, appetite-stimulating hormone secreted by the food-deprived stomach. MBOAT4 can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. MBOAT4 expression, consistent with its function, is mainly in the stomach and intestines. Due to its primary function, MBOAT4 is a candidate for obesity and appetite suppression studies. Function: Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Tissue Specificity: Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues. Similarity: Belongs to the membrane-bound acyltransferase family. SWISS: Q96T53 Gene ID: 619373 Database links: Entrez Gene: 619373 Human Entrez Gene: 234155 Mouse Omim: 611940 Human SwissProt: Q96T53 Human SwissProt: P0C7A3 Mouse Unigene: 627194 Human Unigene: 324008 Mouse
Product Picture	Sample: stomach (mouse) Lysate at 40 ug Primary: Anti- MBOAT4(SL13355R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 55 kD Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MBOAT4) Polyclonal Antibody, Unconjugated (SL13355R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MBOAT4) Polyclonal Antibody, Unconjugated (SL13355R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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