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Product Name TIE1 Chinese Name 血管生成素受体1抗体 Alias TIE1_HUMAN; JKT 14; JTK14; TIE; Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains; Tyrosine protein kinase receptor Tie 1; Tyrosine-protein kinase receptor Tie-1; tyrosine kinase receptor 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1. literatures Specific References (1) | SL1334R has been referenced in 1 publications.[IF=5.57] Zhang C et al. Superparamagnetic iron oxide (SPIO) nanoparticles labeled endothelial progenitor cells (EPCs) administration inhibited heterotopic ossification in rats. Nanomedicine. 2019 Aug 7;21:102078. IHC-P&WB ; Rat.Research Area Tumour Cardiovascular Signal transduction Stem cells Growth factors and hormones Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 123kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Tie1: 851-1000/1138 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.
Function:
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
Subunit:
Heterodimer with TEK/TIE2.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity:
Specifically expressed in developing vascular endothelial cells.
Post-translational modifications:
Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2.
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
SWISS:
P35590
Gene ID:
7075
Database links:
Entrez Gene: 7075 Human
Entrez Gene: 21846 Mouse
Omim: 600222 Human
SwissProt: P35590 Human
SwissProt: Q06806 Mouse
Unigene: 78824 Human
Unigene: 4345 Mouse
Unigene: 13171 Rat
血管生成素-1受体又称上皮生长因子样域酪氨酸激酶1或内皮酪氨酸激酶受体,Tie1在vascular endothelial cell、腺上皮及间质细胞中均有表达,主要定位于细胞质。Product Picture Sample:
A549(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-TIE1 (SL1334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 123 kD
Observed band size: 123 kD
Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TIE1 Polyclonal Antibody, Unconjugated(SL1334R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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