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Product Name phospho-GFAP (Ser38) Chinese Name 磷酸化胶质纤维酸性蛋白抗体 Alias GFAP (phospho S38); p-GFAP (Ser38); Astrocyte; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN. Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Neurobiology Signal transduction Stem cells Cell adhesion molecule Cytoskeleton Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 48kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human GFAP around the phosphorylation site of Ser38: RL(p-S)L Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Function:
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subunit:
Interacts with SYNM. Isoform 3 interacts with PSEN1 (via N-terminus).
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Expressed in cells lacking fibronectin.
Post-translational modifications:
Phosphorylated by PKN1.
DISEASE:
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P14136
Gene ID:
2670
Database links:
Entrez Gene: 2670 Human
Entrez Gene: 14580 Mouse
Omim: 137780 Human
SwissProt: P14136 Human
SwissProt: P03995 Mouse
Product Picture Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- phospho-GFAP (Ser38) (SL13336R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD
Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
Primary Antibody (green line): Rabbit Anti- phospho-GFAP (Ser38) antibody (SL13336R),Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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