Product Name	ZO-1/TJP1
Chinese Name	胞质紧密粘连蛋白1/闭锁小带蛋白1抗体
Alias	ZO1_HUMAN; Tight junction protein ZO-1; TJP1; ZO1; Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1; ZO1 tight junction protein;
literatures	Specific References  (86)     |     SL1329R has been referenced in 86 publications. [IF=20.722] Yuting Qin. et al. Colonic mucus-accumulating tungsten oxide nanoparticles improve the colitis therapy by targeting Enterobacteriaceae. Nano Today. 2021 Aug;39:101234  IF ;  Mouse.   PubMed:10.1016/j.nantod.2021.101234 [IF=13.903] Tang Y et al. Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a "Don't-Eat-Us" Strategy. ACS Nano. 2019 Nov 5.  ICF ;  Mouse.   PubMed:31689086 [IF=11.061] Narendra K. Singh. et al. Coaxial cell printing of a human glomerular model in vitro of the glomerular filtration barrier and its pathophysiology. BIOFABRICATION. 2022 Dec;:  IF ;  Human.   PubMed:36538823 [IF=10.19] Xianqiang Li. et al. Menthol nanoliposomes enhanced anti-tumor immunotherapy by increasing lymph node homing of dendritic cell vaccines. CLIN IMMUNOL. 2022 Sep;:109119  WB ;  Mouse.   PubMed:36109005 [IF=9.933] Hohyeon Han. et al. A Bioprinted Tubular Intestine Model Using a Colon-Specific Extracellular Matrix Bioink. 2021 Nov 16  IF ;  Pig.   PubMed:34747158 [IF=9.229] Xiao Wang. et al. Oral Core–Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy. Acs Appl Mater Inter. 2021;XXXX(XXX):XXX-XXX  IHC ;  Mouse.   PubMed:34261306 [IF=8.856] Zhangcai Liu. et al. A Pathomimetic Colorectal Tumor-on-a-Chip for In Vitro Reconstitution of the Primary Colorectal Cancer. ADV MATER TECHNOL-US. 2023 May;:2200758  ICC ;  Human.   PubMed:10.1002/admt.202200758 [IF=8.025] Yao Li. et al. Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity. INT J BIOL MACROMOL. 2022 Aug;215:113  WB ;  Mouse.   PubMed:35718141 [IF=7.963] Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188  WB ;  Mouse.   PubMed:34875328 [IF=7.546] Jiali Yuan. et al. Effects of metal nanoparticles on tight junction-associated proteins via HIF-1α/miR-29b/MMPs pathway in human epidermal keratinocytes. Part Fibre Toxicol. 2021 Dec;18(1):1-22  IF ;  Human.   PubMed:33740985 [IF=7.31] Longfei Xiao. et al. Curcumin Ameliorates Age-Induced Tight Junction Impaired in Porcine Sertoli Cells by Inactivating the NLRP3 Inflammasome through the AMPK/SIRT3/SOD2/mtROS Signaling Pathway. OXID MED CELL LONGEV. 2023;2023:1708251  WB,IF ;  Pig,Mouse.   PubMed:36846717 [IF=6.706] Zhen-Xing Wang. et al. The Barrier-Enhancing Function of Soluble Yam (Dioscorea opposita Thunb.) Polysaccharides in Rat Intestinal Epithelial Cells, as Affected by the Covalent Se Conjugation. NUTRIENTS. 2022 Jan;14(19):3950  WB ;  Rat.   PubMed:36235602 [IF=6.59] Rongrong Fu. et al. Apigenin remodels the gut microbiota to ameliorate ulcerative colitis.. FRONT NUTR. 2022 Dec;9:1062961-1062961  WB, IHC ;  Mouse.   PubMed:36590200 [IF=6.543] Deng Liangjun. et al. The Attenuation of Chronic Ulcerative Colitis by (R)-salbutamol in Repeated DSS-Induced Mice. Oxid Med Cell Longev. 2022;2022:9318721  WB,IF ;  Mouse.   PubMed:35178163 [IF=6.317] Shuo Yang. et al. Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology. FOOD FUNCT. 2022 Oct;:  IHC ;  Mouse.   PubMed:36326134 [IF=6.317] Zheng Ran. et al. Microbiome–metabolomics analysis reveals the potential effect of verbascoside in alleviating cognitive impairment in db/db mice. FOOD FUNCT. 2023 Mar;:  IHC ;  Mouse.   PubMed:37000613 [IF=6.291] Shaofeng Wu. et al. The neuroprotective effect of curcumin against ATO triggered neurotoxicity through Nrf2 and NF-κB signaling pathway in the brain of ducks. Ecotox Environ Safe. 2021 Dec;228:112965  WB ;  Duck.   PubMed:34775344 [IF=6.208] Zaozao Pan. et al. Protective Effects of Selenium Nanoparticles against Bisphenol A-Induced Toxicity in Porcine Intestinal Epithelial Cells. INT J MOL SCI. 2023 Jan;24(8):7242  WB ;  Pig.   PubMed:37108405 [IF=5.988] Yaying You. et al. Postbiotic muramyl dipeptide alleviates colitis via activating autophagy in intestinal epithelial cells. FRONT PHARMACOL. 2022; 13: 1052644  WB ;  Human, Mouse.   PubMed:36506547 [IF=5.878] Chiara Corpetti. et al. Cannabidiol inhibits SARS-Cov-2 spike (S) protein-induced cytotoxicity and inflammation through a PPARγ-dependent TLR4/NLRP3/Caspase-1 signaling suppression in Caco-2 cell line. 2021 Oct 12  WB ;  Human.   PubMed:34643000 [IF=5.714] Li-Juan You. et al. Schisandrin A ameliorates increased pulmonary capillary endothelial permeability accompanied with sepsis through inhibition of RhoA/ROCK1/MLC pathways. INT IMMUNOPHARMACOL. 2023 May;118:110124  IF,WB ;  Rat,Human.   PubMed:37028276 [IF=5.572] Xu Yang. et al. Deoxynivalenol induces testicular ferroptosis by regulating the Nrf2/System Xc−/GPX4 axis. FOOD CHEM TOXICOL. 2023 May;175:113730  WB ;  Mouse.   PubMed:36925038 [IF=5.546] Yaqi Chang. et al. Glucagon‐like peptide 2 attenuates intestinal mucosal barrier injury through the MLCK/pMLC signaling pathway in a piglet model. J Cell Physiol. 2021 Apr;236(4):3015-3032  IHC ;  Pig.   PubMed:32960454 [IF=5.396] Diqi Yang. et al. Baicalin alleviates endometrial inflammatory injury through regulation of tight junction proteins. FOOD FUNCT. 2022 May;:  WB,IF ;  Sheep,Mouse.   PubMed:35640273 [IF=5.195] Chun Niu. et al. Pulsatilla decoction improves DSS-induced colitis via modulation of fecal-bacteria-related short-chain fatty acids and intestinal barrier integrity. J ETHNOPHARMACOL. 2023 Jan;300:115741  WB ;  Mouse.   PubMed:36162543 [IF=4.927] Junjie Liu. et al. Phenolic Acids from Fructus Chebulae Immaturus Alleviate Intestinal Ischemia-Reperfusion Injury in Mice through the PPARα/NF-κB Pathway. MOLECULES. 2022 Jan;27(16):5227  IHC ;  Mouse.   PubMed:36014464 [IF=4.872] Wanfa Dong. et al. Oral delivery of staphylococcal nuclease ameliorates DSS induced ulcerative colitis in mice via degrading intestinal neutrophil extracellular traps. Ecotox Environ Safe. 2021 Jun;215:112161  IHC ;  Mouse.   PubMed:33812202 [IF=4.651] Masayoshi Ko. et al. Modulation of serotonin in the gut-liver neural axis ameliorates the fatty and fibrotic changes in non-alcoholic fatty liver. Dis Model Mech. 2021 Mar;14(3)  IHC ;  Mouse.   PubMed:33787507 [IF=4.546] Xin-Ran Liu. et al. Radioprotective Effect of Whey Hydrolysate Peptides against γ-Radiation-Induced Oxidative Stress in BALB/c Mice. Nutrients. 2021 Mar;13(3):816  IHC ;  Mouse.   PubMed:33801268 [IF=4.546] Qing Li. et al. Fumonisin B1 Inhibits Cell Proliferation and Decreases Barrier Function of Swine Umbilical Vein Endothelial Cells. Toxins. 2021 Dec;13(12):863  WB ;  Pig.   PubMed:34941701
Research Area	Cell biology  immunology  Signal transduction  Cell adhesion molecule  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Pig,  (predicted: Mouse, Rat, Chicken, Dog, Cow, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	191kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ZO-1: 1551-1702/1733
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017] Function: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Subunit: Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts with HSPA4 and KIRREL1. Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with OCLN, CALM, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with CDC42BPB and MYZAP. Interacts (via PDZ domain) with GJA1. Subcellular Location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, tight junction. Cell junction. Cell junction, gap junction. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells. Tissue Specificity: The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. Post-translational modifications: Phosphorylated. Dephosphorylated by PTPRJ. Similarity: Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 3 PDZ (DHR) domains. Contains 1 SH3 domain. Contains 1 ZU5 domain. SWISS: Q07157 Gene ID: 7082 Database links: Entrez Gene: 7082 Human Entrez Gene: 21872 Mouse Omim: 601009 Human SwissProt: Q07157 Human SwissProt: P39447 Mouse Unigene: 510833 Human Unigene: 4342 Mouse 胞质紧密粘连蛋白1（ZO-1）是多结构域蛋白家族膜结合鸟苷酸激酶的家族成员，在紧密连接蛋白的组成成分中起到对组织分化和器官形成方面起较重要的作用。ZO-1在包括肾、胎盘、血脑屏障等许多组织都有不同的表达，可与紧密连接上的很多Transmembrane protein相互作用。也有学者认为：ZO-1的改变与细胞通透性的增加有关。
Product Picture	Sample: Lane 1: Huvec (Human) Cell Lysate at 30 ug Lane 2: A431 (Human) Cell Lysate at 30 ug Primary: Anti-ZO-1 (SL1329R) at 1/1000 dilution Anti-beta-Actin (SL0061R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 220 kD Observed band size: 220 kD Independently Validated Antibody, image provided by Science Direct, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (SL1329R) at 1:250 followed by conjugation to the secondary antibody. Black line : Positive blank control (293T); Negative blank control (HL60） Green line : Primary Antibody (Rabbit Anti-ZO-1 antibody (SL11320R) ) Orange line：Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647) 293T（Positive）and HL60（Negative control）cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody(SL1329R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). Blank control: 293T Cells(blue). Primary Antibody: Rabbit Anti-ZO-1/AF647 Conjugated antibody (SL1329R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions. Protocol The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (SL1329R-AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.

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