Product Name	GARNL1
Chinese Name	Tuberin样蛋白1抗体
Alias	DKFZp566D133; DKFZp667F074; GAP related interacting partner to E12; GARN L1; GARNL 1; GRIPE; GTPase activating Rap/RanGAP domain like 1; KIAA0884; Tuberin like protein 1; TULIP 1. TULIP1; RGPA1_HUMAN.
Research Area	Cell biology  Neurobiology  Signal transduction  Transporter  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	230 kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GARNL1: 631-730/2036
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GARNL1 is expressed during embryogenesis with E12. During development, GARNL1 expression decreases, persisting at high levels only in neurons of the adult brain. GARNL1 localizes to the cytoplasm where it may play a role regulating GTP hydrolysis of proteins such as Ran and Rap. GARNL1 is imported to the nucleus via dimerization with E12. GARNL1 interacts with the HLH region of E12 and may function to negatively regulate the transcription of E12-dependent downstream target genes. This suggests that at least a portion of the function of GARNL1 is dependent upon its association with E12. GARNL1 may also associate with other HLH proteins and influence a variety of HLH signaling cascades. In adult brain, GARNL1 activity does not involve E12 and therefore it may serve a different function in developed neural tissue. Function: GTPase activating Rap/RanGAP domain-like 1 (GARNL1) is an interacting partner of the transcription factor TCF3/isoform E12, mainly in the developing embryonic forebrain. It may be an important transcriptional regulator of downstream target genes under the control of TCF3/E12, by disrupting HLH dimer formation of TCF3/E12 with other proteins and may be involved in neuronal differentiation. Subunit: Component of the heterodimeric RalGAP1 complex with RALGAPB. Heterodimerization is required for activity. Interacts with the HLH region of TCF3/isoform E12 (By similarity). Subcellular Location: Cytoplasmic and Nuclear. Translocated to the nucleus when associated with TCF3/E12. Tissue Specificity: Widely expressed. Similarity: Contains 1 Rap-GAP domain. SWISS: Q6GYQ0 Gene ID: 253959 Database links: Entrez Gene: 253959 Human Entrez Gene: 56784 Mouse Entrez Gene: 56785 Rat Omim: 608884 Human SwissProt: Q6GYQ0 Human SwissProt: Q6GYP7 Mouse SwissProt: O55007 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GARNL1) Polyclonal Antibody, Unconjugated (SL13286R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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