Product Name	gamma tubulin
Chinese Name	微管蛋白γ/Tubulin γ抗体
Alias	Centrosome Marker; Gamma 1 tubulin; gamma tubulin; gamma-tubulin; Gamma 2 tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma tubulin complex component 1; GCP 1; GCP-1; GCP1; MGC131994; TUBG; TUBG1; TUBG2; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Xgam; TBG1_HUMAN; TBG2_HUMAN; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1.
literatures	Specific References  (1)     |     SL1322R has been referenced in 1 publications. [IF=4.44] Katzenell, Sarah, and David A. Leib. "Herpes simplex virus and interferon signaling induce novel autophagic clusters in sensory neurons." Journal of Virology (2016): JVI-02908.  Mouse.   PubMed:26912623
Research Area	Cell biology  Neurobiology  Signal transduction  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Tubulin gamma-1 chain: 351-451/451
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Centrosome Marker Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton. Function: Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation. Subunit: Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Post-translational modifications: Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome. Similarity: Belongs to the tubulin family. SWISS: P23258 Gene ID: 7283 Database links: Entrez Gene: 27175 Human Entrez Gene: 7283 Human Entrez Gene: 103733 Mouse Entrez Gene: 103768 Mouse Entrez Gene: 252921 Rat Entrez Gene: 680991 Rat Omim: 191135 Human Omim: 605785 Human SwissProt: P23258 Human SwissProt: Q9NRH3 Human SwissProt: P83887 Mouse SwissProt: Q8VCK3 Mouse SwissProt: P83888 Rat Unigene: 279669 Human Unigene: 708059 Human Unigene: 142348 Mouse Unigene: 479145 Mouse Unigene: 154431 Rat 结构蛋白（Structural Proteins） gamma tubulin 微管蛋白是存于中心体的另一种微管蛋白, gamma微管蛋白对微管的形成具有重要作用。gamma微管蛋白通过与β-微管蛋白的相互作用帮助微管的成核反应(nucleation)。即在微管的组装中gamma微管蛋白先形成一个圆或形成钩环结构, γ微管蛋白的这种结构可指导微管蛋白二聚体结合上去并进行微管的组装。gamma微管蛋白在微管的组装中起关键作用。
Product Picture	Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-gamma tubulin Polyclonal Antibody, Unconjugated(SL1322R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (gamma tubulin) polyclonal Antibody, Unconjugated (SL1322R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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