Product Name	FMO5
Chinese Name	二甲基苯胺单加氧酶5抗体
Alias	Dimethylaniline monooxygenase [N oxide forming] 5; Dimethylaniline monooxygenase [N-oxide-forming] 5; Dimethylaniline oxidase 5; FMO 5; FMO5; FMO5_HUMAN; Hepatic flavin containing monooxygenase 5; Hepatic flavin-containing monooxygenase 5.
Research Area	Tumour  Cell biology  Signal transduction  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	60kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FMO5: 251-350/533
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The Flavin containing monooxygenase family consists of five gene products, FMO1-5, that are major enzymatic oxidants involved in the metabolism of various therapeutics. Localizing to microsomal and endoplasmic reticulum membranes, FMO5 (flavin containing monooxygenase 5), also known as dimethylaniline monooxygenase [N-oxide-forming] 5, hepatic flavin-containing monooxygenase 5 or dimethylaniline oxidase 5, is a 533 amino acid protein belonging to the FMO family. Expressed in adult and fetal liver, FMO5 is unlike other FMO family members because it does not function as a drug-metabolizing enzyme. FMO5 binds FAD as a cofactor and is encoded by a gene located on human chromosome 1q21.1. Function: In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme. Subcellular Location: Microsome membrane. Endoplasmic reticulum membrane. Tissue Specificity: Expressed in fetal and adult liver. Similarity: Belongs to the FMO family. SWISS: P49326 Gene ID: 2330 Database links: Entrez Gene: 2330 Human Entrez Gene: 14263 Mouse Entrez Gene: 246248 Rat Omim: 603957 Human SwissProt: P49326 Human SwissProt: P97872 Mouse SwissProt: Q8K4C0 Rat Unigene: 642706 Human Unigene: 385180 Mouse Unigene: 7038 Rat
Product Picture	Protein: liver(mouse)lyates at 40ug; Primary: Anti-FMO5 (SL13187R) at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000; ECL excitated the fluorescence; Predicted band size : 60 kD Observed band size :60 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FMO5 Polyclonal Antibody, Unconjugated(SL13187R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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