Product Name	Ferredoxin Reductase
Chinese Name	铁氧化还原蛋白还原酶抗体
Alias	adrenodoxin reductase; ADRO_HUMAN; ADXR; AR; FDXR; Ferredoxin NADP(+) reductase; Ferredoxin reductase; Ferredoxin--NADP(+) reductase; mitochondrial; NADPH adrenodoxin oxidoreductase mitochondrial; NADPH:adrenodoxin oxidoreductase.
Research Area	Tumour  Cell biology  immunology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	50kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Ferredoxin Reductase: 401-491/491
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Adrenodoxin Reductase (ADX Reductase) is a mitochondrial flavoprotein that receives electrons from NADPH and thereby initiates the electron-transport chain serving mitochondrial cytochromes P450. ADX Reductase participates in cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-? hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney and sterol C-27 hydroxylation in the liver. Alternate splicing of ADX Reductase produces two isoforms. Human ADX Reductase maps to human chromosome 17q24-q25. Function: Serves as the first electron transfer protein in all the mitochondrial P450 systems. Including cholesterol side chain cleavage in all steroidogenic tissues, steroid 11-beta hydroxylation in the adrenal cortex, 25-OH-vitamin D3-24 hydroxylation in the kidney, and sterol C-27 hydroxylation in the liver. Subunit: Monomer. Interacts directly with FDX1. Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the ferredoxin--NADP reductase type 1 family. SWISS: P22570 Gene ID: 2232 Database links: Entrez Gene: 2232 Human Entrez Gene: 14149 Mouse Entrez Gene: 79122 Rat Omim: 103270 Human SwissProt: P22570 Human SwissProt: Q61578 Mouse SwissProt: P56522 Rat Unigene: 69745 Human Unigene: 4719 Mouse Unigene: 10860 Rat
Product Picture	Blank control:Mouse kidney. Primary Antibody (green line): Rabbit Anti-Ferredoxin Reductase antibody (SL13154R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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