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Product Name LYVE-1 Chinese Name 淋巴管内皮透明质酸受体抗体 Alias LYVE1; lymphalic vessel endotheilial hyaluronan receptor 1; CRSBP 1; CRSBP1; extracellular link domain containing 1; extracellular link domain-containing 1; HAR; hyaluronic acid receptor; Cell surface retention sequence-binding protein 1; CRSBP-1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; Lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1_MOUSE; Lymphatic endothelium specific hyaluronan receptor; LYVE 1; LYVE-1; XLKD1. literatures Specific References (5) | SL1311R has been referenced in 5 publications.[IF=3.3] Merigo et al. Glucose transporter expression in the human colon. (2018) World.J.Gastroenterol. 24:775-793 IHC-P ; Human.[IF=2.11] Zhang, Chun, et al. "MTDH/AEG-1-based DNA vaccine suppresses metastasis and enhances chemosensitivity to paclitaxel in pelvic lymph node metastasis."Biomedicine & Pharmacotherapy (2015). IHC-P ; Mouse.[IF=1.935] Hayashi T et al. Characteristics of Leiomyosarcoma: Induction of Hematogenous Metastasis by Isolated Uterine Mesenchymal Tumor Stem-like Cells Anticancer Res. 2020 Mar;40(3):1255-1265. IHC ; human.[IF=1.68] Sun, Y., et al. "A Reproducible In-vivo Model of Lymphatic Malformation in Rats." Journal of comparative pathology 145.4 (2011): 390-398. IHC-P ; Rat.[IF=0] Michurina et al. Linagliptin alleviates fatty liver disease in diabeticdb/dbmice. (2016) World.J.Diabetes. 7:534-546 IHC ; Mouse.Research Area Cardiovascular Cell biology immunology Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 32kDa Cellular localization cytoplasmic The cell membrane Extracellular matrix Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse LYVE-1 terminus: 231-318/318 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. Recently a number of interesting molecules have been identified that may be exploited as markers for lymphatic endothelium, including the hyaluronan receptor LYVE1, PALE, VEGFR3, podoplanin. LYVE1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.
Function:
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
Subunit:
Homodimer; disulfide-linked. Interacts with PDGFB and IGFBP3. Forms a transient ternary complex with PDGFB and PDGFRB in TGN.
Subcellular Location:
Membrane; Single-pass type I membrane protein. Note=Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
Post-translational modifications:
O-glycosylated.
Similarity:
Contains 1 Link domain.
SWISS:
Q9Y5Y7
Gene ID:
114332
Database links:Entrez Gene: 10894 Human
Entrez Gene: 114332 Mouse
Omim: 605702 Human
SwissProt: Q9Y5Y7 Human
SwissProt: Q8BHC0 Mouse
Unigee: 246769 Human
Unigene: 655332 Human
Unigene: 396078 Mouse
淋巴管Maker
LYVE-1是一种特异性氨基葡聚糖透明质酸受体,均匀分布在淋巴管的管腔面和基底膜,目前多用于Tumour淋巴转移方面的研究。
淋巴管endothelial cells透明质酸受体1(LYVE1)LYVE1是位于淋巴管endothelial cells上的含有322个氨基酸残基的膜蛋白。与CD44glycoprotein同源,均匀分布于淋巴管内外腔面,可与Extracellular matrix的葡萄糖胺聚糖透明质酸结合,具有从组织摄取透明质酸盐并转运至淋巴液的作用。Product Picture Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Spleen tissue lysates
Lane 3: Human Huvec cell lysates
Lane 4: Human K562 cell lysates
Primary: Anti- LYVE-1 (SL1311R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 58 kD
Tissue/cell: Human oral squamous cell carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LYVE-1 Polyclonal Antibody, Unconjugated(SL1311R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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